邓益琴;张亚秋;林梓阳;冯娟;程长洪;马红玲;刘广鑫;苏友禄;

• 1:中国水产科学研究院南海水产研究所/农业农村部南海渔业资源开发利用重点实验室
• 2:上海海洋大学水产与生命学院
• 3:海南大学海洋学院
• 4:广东省水环境与水产品安全工程技术研究中心/仲恺农业工程学院动物科技学院

摘要(Abstract)：

【目的】研究哈维弧菌（Vibrioharveyi）345的GTP结合蛋白编码基因flhF和未知功能基因CU052＿26825的生物学功能。【方法】利用同源重组技术构建V. harveyi 345的flhF和CU052＿26825的缺失突变株，比较野生株和突变株的生长、运动性、胞外酶活性、耐药性等生物学特性以及对花鲈（Lateolabraxjaponicus）的毒性。【结果】基因flhF和CU052＿26825的缺失均不影响菌株的生长、运动性、胞外蛋白酶活性、对H2O2和Cu2+的压力感应、对铁的吸收利用、对大多数被测抗生素的抗性以及对花鲈的毒性；但flhF和CU052＿26825缺失后对氯霉素和氟苯尼考两种氯霉素类抗生素均更加敏感。此外，flhF缺失后，细菌生物膜形成能力显著增强。【结论】flhF和CU052＿26825均参与调控哈维弧菌对氯霉素类抗生素的耐药性，flhF还参与调控哈维弧菌生物膜形成。

关键词(KeyWords)： 哈维弧菌;基因敲除;flhF;CU052＿26825;生物学特性;氯霉素类耐药性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31902415);; 中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助（2022GH03）;中国水产科学研究院基本科研业务费专项资金（2020XT0407）;中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助（2020TS04）;; 国家重点研发计划资助（2019YFD0900105);; 广东省自然科学基金(2019A1515011833)

作者(Authors): 邓益琴;张亚秋;林梓阳;冯娟;程长洪;马红玲;刘广鑫;苏友禄;

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