韦福佳,陆家昌,赖俊翔,李杰,庄军莲,张萍,李锐龙

• 1:广西大学资源环境与材料学院
• 2:广西科学院广西北部湾海洋研究中心/广西近海海洋环境科学重点实验室

摘要(Abstract)：

【目的】研究盐度对球形棕囊藻（Phaeocystis globosa）光合活性的影响，进一步认识该物种时空分布格局的形成，为赤潮预警预报提供基础数据。【方法】将2.00×10~4mL~(-1)的单细胞球形棕囊藻分别在0、5、10、15、20、25、30、35和40等9个盐度下培养7 d，每天用流式计数法测定细胞丰度，用叶绿素荧光仪测定叶绿素荧光参数。【结果与结论】球形棕囊藻最适生长盐度为35，藻细胞在盐度25～40下生长良好，在盐度15～20下生长缓慢，在盐度为0时全部死亡，叶绿素a荧光值下降为0，在盐度10和5时停止生长并出现大量死亡。藻细胞光合系统Ⅱ在15～40盐度条件下功能正常；0～10盐度条件下，遭受不同程度的破坏，其中0盐度条件下1 d内遭受不可逆破坏，最大光能转化效率（F_v/F_m）、实际光能转化效率（Φ_(PSⅡ)）、初始斜率（α）、最大相对电子传递速率（rETR_(max)）、半饱和照度（I_k）、非光化学淬灭系数（NPQ）和光化学淬灭系数（qP）急剧下降，并不可恢复；5盐度组也在1 d内遭受严重损伤，存活藻细胞在4 d后光合系统部分恢复，其F_v/F_m、Φ_(PSⅡ)、α、NPQ和qP值迅速降低后逐渐上升，但多未恢复到正常水平；10盐度条件下4 d后胁迫效应方出现，其F_v/F_m、Φ_(PSⅡ)、α值在4 d后下降，7 d时显著低于15～40盐度处理（P<0.05）。为修复受损的光合系统，细胞对强光的耐受能力提高，7 d时5与10盐度组的rETR_(max)和I_k值显著高于其他各组（P<0.05）。

关键词(KeyWords)： 球形棕囊藻;盐度;生长;叶绿素荧光参数;光合活性;光合系统

Abstract:

Keywords:

基金项目(Foundation): 广西创新驱动发展专项（AA17202020）

作者(Author): 韦福佳,陆家昌,赖俊翔,李杰,庄军莲,张萍,李锐龙

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