用冷热休克诱导四倍体近江牡蛎TETRAPLOIDY INDUCED BY COLD AND HEAT SHOCK IN OSTREA RIVULARIS
容寿柏,李一民,刘绍琼,李寿武,范伟,黄雨青
摘要(Abstract)：
当近江牡蛎授精卵发育至第一次卵裂前3min或个别受精卵开始出现第一次卵裂时,分别用热体克(37℃,39℃,41℃)和冷休克(8℃,10℃,12℃)处理3min诱导四倍体。测定39℃热休克组胚胎早期的四倍体诱导率为28％,10℃冷休克组胚胎早期的四倍体诱导率为30%。温度过高(如42℃以上)可导致卵的高死亡率。
关键词(KeyWords)： 热休克;冷休克;四倍体;近江牡蛎
基金项目(Foundation):
作者(Author): 容寿柏,李一民,刘绍琼,李寿武,范伟,黄雨青
参考文献(References)：
- [1] 于瑞海,贝类三倍体倍育技术.海洋科学,1989(4) :69-71
- [2] 容寿柏等,用冷热休克诱导三倍体近江牡蛎.湛江水产学院学报,1990;10(2) :32-86
- [3] Ahmed.M., and Sparks, A.K., 1970, Chromosome number, structure and autosomal polymerphism in the marine mussels mytilus edulis and M. Californianus Biol. Bull.,138 (1) ; 1-33
- [4] Downing, S.L., and Allen, S.K.Jr., 1987, Induced triploidy in the Pacific oyster, Crassostrea gigas: optimal treatment with cytochalasin B depend on temperature, Aquaculture, 61: 1-15
- [5] Okamoto, A. and Arimoto, B., 1986． Chromosome of Corbicula Junponica, C'. Sandai and C. (Corbiculina) leana (Bivalvia: Corbiculidae). Venus, 45 (3) : 194-202．
- [6] Quillet, E., and P.J. Panelay, 1986, Triploidy induction by thermal shocks in the Japanese oyster, Crassostrea gigas, Aquaculture, 57: 271-279．
- [7] Stanley, J.G., S.K. Jr. Allen, and H. Hidu, 1981, Polyploidy induced in the American oyster, Crassostrea Virginica, with cytochlasin B, Aquaculture, 23: 1-10
- [8] Stanley, J.G., H. Hidu, and S. K. Jr. Allen, 1984, Growth of American oyster increased by polyploidy induced by blocking meiosis I but not meiosis II, Aquaculture, 37: 147-155．
- [9] Yamamoto, S. and Sugawara, Y., 1988． Induced triploidy in the mussel, Mytilus, edulis by temperature shock. Aquaculture, 72: 21-29．