汪兰,吴伟伦,石柳,佀国涵,袁家富,赵书军,彭成林

• 1:湖北省农业科学院
• 2:湖北省农业科技创新中心农产品加工研究分中心
• 3:国家土壤质量洪山观测实验站

摘要(Abstract)：

【目的】探明光叶紫花苕子（Vicia villosa Rothvar）和箭筈豌豆（Vicia sativa L.）这两种豆科绿肥饲料添加对克氏原螯虾（Procambarus clarkii）生长性能及虾肉品质的影响。【方法】用五种饲料处理组（T1组，光叶紫花苕子；T2组，箭筈豌豆；T3组，专用饲料；T4组，光叶紫花苕子+专用饲料；T5组，箭舌豌豆+专用饲料）饲喂克氏原螯虾60 d后，分析豆科绿肥添加对其生长性能、理化性质和组织结构的影响。【结果】光叶紫花苕子和箭舌豌豆中蛋白质质量分数为27.80%和25.00%，粗纤维质量分数均为22.60%，天冬氨酸和亮氨酸含量相对较高。饲喂60 d后，与只饲喂专用饲料（T3组）相比，不同组别克氏原螯虾成活率无显著变化（76.7%～80.0%,P> 0.05），但T1、T4和T5组增重率分别上升8.13%、32.92%和27.95%，且差异显著（P <0.05）,T2组得肉率显著上升9.29%(P <0.05);T4和T5组加压失水率分别显著下降20.6%和18.8%(P <0.05)，除T1组L*值下降外，其他组别色泽皆有一定程度上升，T4和T5组的虾肉肌纤维间隙减少，虾肉更加紧密。【结论】相比于单独饲喂专用饲料或豆科绿肥，混合饲喂效果更佳，且箭舌豌豆+专用饲料混合饲喂比光叶紫花苕子+专用饲料混合饲喂的克氏原螯虾生长性能和虾肉品质更佳。

关键词(KeyWords)： 光叶紫花苕子;箭筈豌豆;克氏原螯虾;生长性能;肌肉品质

Abstract:

Keywords:

基金项目(Foundation): 湖北省重点研发计划项目（2022BBA0044,2021BBA075）;; 水产品智能化加工工程技术研发及装备集成项目（2023BEB030）

作者(Author): 汪兰,吴伟伦,石柳,佀国涵,袁家富,赵书军,彭成林

参考文献(References)：

• [1]于秀娟,郝向举,杨霖坤,等.中国小龙虾产业发展报告(2023)[J].中国水产, 2023(7):26-31.
• [2]宋光同,王芬,蒋业林,等. 5种饲料组合投喂下克氏原螯虾生长及肌肉品质的比较分析[J].江苏农业科学, 2022,50(7):172-177.
• [3] CHEN C Z, XU C, YANG X L, et al. Growth, antioxidant capacity, intestine histology and lipid metabolism of juvenile red claw crayfish, Cherax quadricarinatus, fed different lipid sources[J]. Aquaculture Nutrition, 2021, 27(1):261-273.
• [4] QIAN D W, YANG X L, XU C, et al. Growth and health status of the red claw crayfish, Cherax quadricarinatus, fed diets with four typical plant protein sources as a replacement for fish meal[J]. Aquaculture Nutrition, 2021, 27(3):795-806.
• [5] TAN Q, SONG D, CHEN X, et al. Replacing fish meal with vegetable protein sources in feed for juvenile red swamp crayfish, Procambarus clarkii:effects of amino acids supplementation on growth and feed utilization[J]. Aquaculture Nutrition, 2018, 24(2):858-864.
• [6] ZHU L, KONG Y M, CHANG X L, et al. Effects of two fish-derived probiotics on growth performance, innate immune response, intestinal health, and disease resistance of Procambarus clarkii[J]. Aquaculture, 2023, 562:738765.
• [7] YANG Y, XU W Y, DU X L, et al. Effects of dietary melatonin on growth performance, antioxidant capacity, and nonspecific immunity in crayfish, Cherax destructor[J]. Fish&Shellfish Immunology, 2023, 138:108846.
• [8] SHEHATA A I, ALHOSHY M, WANG T, et al. Dietary supplementations modulate the physiological parameters,fatty acids profileand the growth of red claw crayfish(Cherax quadricarinatus)[J]. Journal of Animal Physiology and Animal Nutrition, 2023, 107(1):308-328.
• [9]金鸿浩,李雨,李哲,等.克氏原螯虾营养需求与饲料研究进展[J].水产科学, 2023, 42(5):891-900.
• [10] MARTINOLI M, PULCINI D, VEROLI M, et al. Effects of Portulaca oleracea whole diet on pigments and fatty acids profiles of juvenile red swamp crayfish(Procambarus clarkii Girard, 1852)under laboratory conditions[J]. Aquaculture Research, 2021, 52(11):5180-5189.
• [11] WU L M, HAN G M, ZHANG J H, et al. Feeding effect of Alternanthera philoxeroides and Elodea nuttallii on crayfish(Procambarus clarkii)growth, hepatopancreas enzyme activity,and muscle quality[J]. Aquaculture International, 2023:1-16.
• [12]彭飞,向蕊,陈建明,等.中草药添加剂在水产养殖中的研究与应用进展[J].饲料研究, 2023, 46(12):168-172.
• [13]张泽茹,赵玉全,董可,等.植物提取物促动物生长作用机制的研究进展[J].动物营养学报, 2023, 35(8):4850-4859.
• [14] MAHIDOL C, KITTAKOOP P, PRACHYAWARAKORN V,et al. Recent investigations of bioactive natural products from endophytic, marine-derived, insect pathogenic fungi and Thai medicinal plants.[J]. Pure and Applied Chemistry,2014, 86(6):979-993.
• [15]尚益民.我国豆科绿肥共生根瘤菌多样性分析与优势菌种资源挖掘[D].郑州:郑州轻工业大学, 2020.
• [16] HUANG Y F, GAO X L, NAN Z B, et al. Potential value of the common vetch(Vicia sativa L.)as an animal feedstuff:a review[J]. Journal of Animal Physiology and Animal Nutrition, 2017, 101(5):807-823.
• [17]程园,封朝壁,张文,等.豆科绿肥草粉对生长育肥猪生产性能和消化道形态的影响[J].甘肃农业大学学报,2000, 35(2):222-226.
• [18]刘永钢,徐载春,王洪桐.不同比例光叶紫花苕草粉饲喂生长肥育猪的效果[J].中国畜牧杂志, 1992, 28(1):3-7.
• [19] PALSANIYA D R, KUMAR S, SHIVKUMAR B G, et al.Stylosanthes production, processing and utilization technologies for animal production-a review[J]. Animal Nutrition and Feed Technology, 2017, 17(3):501.
• [20] CICEK H, MARTENS J R T, BAMFORD K C, et al. Forage potential of six leguminous green manures and effect of grazing on following grain crops[J]. Renewable Agriculture and Food Systems, 2015, 30(6):503-514.
• [21]石钢鹏,高天麒,阙凤,等.不同捕捞月份和规格克氏原螯虾品质比较[J].食品工业科技, 2022, 43(19):53-60.
• [22]陈东清,汪兰,熊光权,等.不同规格小龙虾原料加工特性研究[J].食品安全质量检测学报, 2019, 10(8):2156-2161.
• [23] YAO X Z, LIN Y Y, SHI M L, et al. Effect of Schizochytrium limacinum supplementation to a low fish-meal diet on growth performance, lipid metabolism, apoptosis, autophagy and intestinal histology of Litopenaeus vannamei[J]. Frontiers in Marine Science, 2022, 9:1090235.
• [24]杜雪莉,张凌晶,杨欣怡,等. 4种饲料养殖小龙虾营养分析及品质评价[J].食品安全质量检测学报, 2022, 13(2):576-584.
• [25]夏思瑶,万金娟,孟祥龙,等.饲料中蛋白质水平对克氏原螯虾生长性能及肌肉品质的影响[J].水产养殖, 2021,42(3):6-12.
• [26] WANG C, SHI G P, QUE F, et al. Effect of microstructure and chemical proximate composition on mechanical properties of Procambarus clarkii shell[J]. LWT, 2022, 165:113731.
• [27]崔燕,朱麟,尚海涛,等.低温高湿解冻对南美白对虾保水性及肌原纤维蛋白生化特性的影响[J].食品科学技术学报, 2020, 38(2):81-89.
• [28] ZHU K, YAN W, DAI Z Y, et al. Astaxanthin extract from shrimp(Trachypenaeus curvirostris)by-products improves quality of ready-to-cook shrimp surimi products during frozen storage at-18℃[J]. Foods, 2022, 11(14):2122.
• [29] LI D Y, YUAN Z, LIU Z Q, et al. Effect of oxidation and Maillard reaction on color deterioration of ready-to-eat shrimps during storage[J]. LWT, 2020, 131:109696.
• [30] AMBATI R R, PHANG S M, RAVI S, et al. Astaxanthin:sources, extraction, stability, biological activities and its commercial applications:a review[J]. Marine Drugs, 2014,12(1):128-152.
• [31]杨肖杰,黄卉,李来好,等.南美白对虾酶促剥壳工艺优化及其对虾仁品质的影响[J].食品与发酵工业, 2021, 47(11):119-125.
• [32]祁雪儿,毛俊龙,姚慧,等.蛋白质氧化对中华管鞭虾肌肉品质特性的影响[J].食品科学, 2021, 42(18):15-21.
• [33] CAI M L, ZHANG Y, ZHU J Q, et al. Intervention of re-feeding on growth performance, fatty acid composition and oxidative stress in the muscle of red swamp crayfish(Procambarus clarkii)subjected to short-term starvation[J].Aquaculture, 2021, 545:737110.