方玲玲,陈刚,王忠良,汤保贵,张健东,黄建盛,周晖

• 1:广东海洋大学水产学院
• 2:广东省普通高校南海水产经济动物增养殖重点实验室
• 3:广东省水产经济动物病原生物学及流行病学重点实验室

摘要(Abstract)：

采用RACE-PCR克隆卵形鲳鲹(Trachinotus ovatus)过氧化物酶体增殖物激活受体(peroxisome proliferator activated receptors-α,PPARα)基因的c DNA序列全长,并应用生物信息学方法分析其编码蛋白质的理化性质和结构特征。结果表明:卵形鲳鲹PPARα基因(Gen Bank登录号KP893147)c DNA全长1 930 bp,开放阅读框(ORF)为1 425 bp,共编码474个氨基酸,其编码的蛋白质为不稳定蛋白,无信号肽和跨膜结构,二级结构由α螺旋、β转角、伸展片段和无规则卷曲组成,且α螺旋占较大比例;预测显示,该蛋白有PPARs基因家族典型的DNA结合区(DBD)和配体结合区(LBD);序列对比表明,卵形鲳鲹PPARα基因与尼罗罗非鱼(Oreochromis niloticus)、花鲈(Lateolabrax japonicas)、大黄鱼(Larimichthys crocea)、金头鲷(Sparus aurata)、军曹鱼(Rachycentron canadum)等有较高的同源性(81%~89%);蛋白系统进化树分析显示,在人(Homo sapiens)、鼠(Mus musculus)、鸭(Gallus gallus)、花鲈、大黄鱼、军曹鱼等动物中,卵形鲳鲹的PPARα蛋白与军曹鱼的进化关系最为密切(94%),与人(68%)、鼠(68%)、鸭(67%)等的同源性较低。荧光定量分析显示,卵形鲳鲹PPARαm RNA在脑、肾脏、肠、脾脏等组织表达水平较高,其次是皮肤、肌肉,在心脏、肝脏中表达量较低。

关键词(KeyWords)： 卵形鲳鲹;过氧化物酶体增殖物激活受体α(PPARα);基因;克隆;cDNA末端快速扩增(RACE);生物信息学;组织表达

Abstract:

Keywords:

基金项目(Foundation): 国家海洋公益性行业科研专项(201205028);; 广东省海洋经济创新发展区域示范专项(GD2012-A01-007,GD2012-A02-003);; 广东省教育厅创新计划专项(2012KJCX0063);; 广西科技厅科技计划(桂科攻1222013-2)

作者(Author): 方玲玲,陈刚,王忠良,汤保贵,张健东,黄建盛,周晖

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