王成强,李宝山,孙永智,王晓艳,郝甜甜,相智巍,刘财礼,王佩锋,王际英

• 1:山东省海洋资源与环境研究院/山东省海水渔用饲料工程技术研究中心/水生动物营养与饲料研发创新示范平台/山东省海洋生态修复重点实验室/烟台市海珍品质量安全控制与精深加工重点实验室
• 2:上海海洋大学水产与生命学院

摘要(Abstract)：

【目的】探究饲料中添加包膜丁酸钠对大规格刺参（Apostichopus japonicus）生长性能、消化和抗氧化能力及相关基因表达的影响，为丁酸钠在刺参养成阶段生产中的合理利用提供依据。【方法】选用初始体质量为（55.96±0.20）g的刺参为研究对象，通过在基础饲料中添加质量分数为0、0.05%、0.10%、0.20%、0.40%、0.60%的包膜丁酸钠（丁酸钠有效质量分数≥75%），制成6组等氮等脂的实验饲料，其中未添加组为对照组，养殖周期为8周。【结果】实验结果表明，刺参存活率在不同实验组间无显著差异（P> 0.05），增重率（WGR）及特定生长率（SGR）均在0.40%添加组达到最高水平，显著高于对照组、0.05%和0.10%添加组（P <0.05）；刺参肠道蛋白酶和脂肪酶活性均在0.40%添加组达到最高水平，同0.60%添加组无显著差异（P> 0.05），显著高于对照组（P <0.05）。另外，肠道淀粉酶活性在不同实验组间无显著差异（P> 0.05）；刺参肠道免疫能力方面，添加量在0.20%以上时，肠道中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性和总抗氧化能力（T-AOC）均显著高于对照组（P <0.05），而肠道丙二醛（MDA）含量呈相反的变化趋势（P <0.05）；刺参肠道NF-κB转录因子（Rel）相对表达量均在0.40%添加组组达到最高值，显著高于对照组（P <0.05），同时肠道NF-κB转录因子（P105）相对表达量同Rel呈相反变化趋势（P <0.05）。刺参肠道溶菌酶（lysozyme）相对表达量在0.40%添加组达到最高值，显著高于对照组和0.60%添加组（P <0.05）。【结论】在本研究条件下，饲料中添加0.40%的包膜丁酸钠对大规格刺参生长性能、消化能力和抗氧化能力起到一定的促进作用。丁酸钠可以通过调节NF-κB信号通路相关因子影响刺参的抗炎水平，进而对机体的免疫能力产生积极影响。

关键词(KeyWords)： 刺参;包膜丁酸钠;生长性能;抗氧化能力;基因表达

Abstract:

Keywords:

基金项目(Foundation): 山东省现代农业产业技术体系刺参产业技术体系(SDAIT-22-06);; 海洋生物资源的开发与利用项目（220-0110-JBN-54EY）

作者(Author): 王成强,李宝山,孙永智,王晓艳,郝甜甜,相智巍,刘财礼,王佩锋,王际英

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