孙东,陈琪,朱博,段美娜

• 1:广东海洋大学化学与环境学院
• 2:暨南大学生命科学技术学院
• 3:西南科技大学生命科学与工程学院

摘要(Abstract)：

【目的】研究红树植物海芒果果实水提物及其主要抑藻物质原儿茶酸对典型赤潮藻米氏凯伦藻生长、光合系统PSⅡ的抑制作用及其机制。【方法】将米氏凯伦藻分别接种于含有海芒果果实水提物和原儿茶酸的f/2培养基中,接种量4.5×104 cells·L~(-1)。计算海芒果果实水提物和原儿茶酸对米氏凯伦藻的抑制效率,并分析米氏凯伦藻光合系统Ⅱ中JIP-test和电子传递链在胁迫条件下的响应机制。【结果】海芒果果实水提物及其主要抑藻物质原儿茶酸对米氏凯伦藻的生长均有显著的抑制作用,可显著抑制米氏凯伦藻光合系统Ⅱ中"J-I-P"相的增长,可引发单位激发界面能量通量相关指标(ABS/CS_0、DI_0/CS_0、ET_0/CS_0、TR_0/CS_0)的下降,导致光合系统Ⅱ电子的传递、转移受阻。【结论】海芒果果实水提物和原儿茶酸有用于防控赤潮藻米氏凯伦藻的潜力。

关键词(KeyWords)： 海芒果果实;米氏凯伦藻;有害藻华;光合系统Ⅱ

Abstract:

Keywords:

基金项目(Foundation): 广东海洋大学博士科研启动项目(060302122009);; 中国博士后自然科学基金(2018M633276)

作者(Author): 孙东,陈琪,朱博,段美娜

参考文献(References)：

• [1] HEISLER J, GLIBERT P M, BURKHOLDER J M, et al.Eutrophication and harmful algal blooms:a scientific consensus[J]. Harmful Algae, 2008, 8(1):3-13.
• [2]陈东,黄翔鹄,李长玲,等.侧孢短芽孢杆菌对铜绿微囊藻藻际微生物群落的影响[J].广东海洋大学学报,2021, 41(2):10-17.
• [3] BROWN A R, LILLEY M, SHUTLER J, et al. Assessing risks and mitigating impacts of harmful algal blooms on mariculture and marine fisheries[J]. Reviews in Aquaculture, 2020, 12(3):1663-1688.
• [4] LEE C K, PARK T G, PARK Y T, et al. Monitoring and trends in harmful algal blooms and red tides in Korean coastal waters, with emphasis on Cochlodinium polykrikoides[J]. Harmful Algae, 2013, 30:S3-S14.
• [5]卓鑫.近十年福州沿海赤潮的基本特征研究[J].海洋预报, 2018, 35(4):34-40.
• [6] BRAND L E, CAMPBELL L, BRESNAN E. Karenia:The biology and ecology of a toxic genus[J]. Harmful Algae, 2012, 14:156-178.
• [7] GALLARDO-RODRíGUEZ J J, ASTUYA-VILLALóN A, LLANOS-RIVERA A, et al. A critical review on control methods for harmful algal blooms[J]. Reviews in Aquaculture, 2019, 11(3):661-684.
• [8] CHEN Q, ZHU B, SUN D, et al. The effect of protocatechuic acid on the phycosphere in harmful algal bloom species Scrippsiella trochoidea[J]. Aquatic Toxicology, 2020, 227:105591.
• [9] SUN Y Y, MENG K, SU Z X, et al. Isolation and purification of antialgal compounds from the red alga Gracilaria lemaneiformis for activity against common harmful red tide microalgae[J]. Environmental Science and Pollution Research, 2017, 24(5):4964-4972.
• [10] CHEN Q, SUN D, FANG T, et al. In vitro allelopathic effects of compounds from Cerbera manghas L. on three Dinophyta species responsible for harmful common red tides[J]. Science of the Total Environment, 2021, 754:142253.
• [11] ANTONY F M, WASEWAR K. Reactive extraction:a promising approach to separate protocatechuic acid[J].Environmental Science and Pollution Research International, 2020, 27(22):27345-27357.
• [12] KAKKAR S, BAIS S. A review on protocatechuic Acid and its pharmacological potential[J]. ISRN Pharmacology, 2014, 2014:952943.
• [13] LI L. Effect of combined pollution of Cd and B[a]P on photosynthesis and chlorophyll fluorescence characteristics of wheat[J]. Polish Journal of Environmental Studies, 2015, 24:157-163.
• [14] WANG J, ZHU J Y, GAO Y N, et al. Toxicity of allelochemicals released by submerged macrophytes on phytoplankton[J]. Allelopathy Journal, 2013, 31(1):199-209.
• [15] MEHTEROV N, BALAZADEH S, HILLE J, et al.Oxidative stress provokes distinct transcriptional responses in the stress-tolerant atr7 and stress-sensitive loh2 Arabidopsis thaliana mutants as revealed by multi-parallel quantitative real-time PCR analysis of ROS marker and antioxidant genes[J]. Plant Physiology and Biochemistry, 2012, 59:20-29.
• [16] WRZACZEK M, BROSCHéM, KANGASJ?RVI J.ROS signaling loops-production, perception,regulation[J]. Current Opinion in Plant Biology, 2013,16(5):575-582.
• [17] AHAMMED G J, YUAN H L, OGWENO J O, et al.Brassinosteroid alleviates phenanthrene and Pyrene phytotoxicity by increasing detoxification activity and photosynthesis in tomato[J]. Chemosphere, 2012, 86(5):546-555.
• [18] LI Q S, LU Y L, SHI Y J, et al. Combined effects of cadmium and fluoranthene on germination, growth and photosynthesis of soybean seedlings[J]. Journal of Environmental Sciences, 2013, 25(9):1936-1946.
• [19] SUN D, HE N, CHEN Q, et al. Effects of lanthanum on the photosystem II energy fluxes and antioxidant system of Chlorella vulgaris and Phaeodactylum tricornutum[J].International Journal of Environmental Research and Public Health, 2019, 16(12):2242.
• [20] KALAJI H M, RA?KOVáL, PAGANOVáV, et al.Can chlorophyll-a fluorescence parameters be used as bio-indicators to distinguish between drought and salinity stress in Tilia cordata Mill?[J]. Environmental and Experimental Botany, 2018, 152:149-157.
• [21] SUN ZW, TIAN F, DUAN LY, et al. Allelopathic effects of mangrove plant Bruguiera gymnorrhiza on microalgae[J]. Allelopathy Journal, 2012, 30(2):291-298.
• [22] ZHU JY, XIAO H, CHEN Q, et al. Growth inhibition of Phaeocystis globosa induced by luteolin-7-o-glucuronide from seagrass Enhalus acoroides[J]. International Journal of Environmental Research and Public Health,2019, 16(14):2615.
• [23] ZHAO M, XIAO H, SUN D, et al. Investigation of the inhibitory effects of mangrove leaves and analysis of their active components on Phaeocystis globosa during different stages of leaf age[J]. International Journal of Environmental research and Public Health, 2018, 15(11):2434.
• [24] TAN K T, HUANG Z Q, JI R B, et al. A review of allelopathy on microalgae[J]. Microbiology(Reading,England), 2019, 165(6):587-592.
• [25] SINGH-TOMAR R, JAJOO A. Alteration in PS II heterogeneity under the influence of polycyclic aromatic hydrocarbon(fluoranthene)in wheat leaves(Triticum aestivum)[J]. Plant Science, 2013, 209:58-63.
• [26] AKSMANN A, TUKAJ Z. Intact anthracene inhibits photosynthesis in algal cells:a fluorescence induction study on Chlamydomonas reinhardtii cw92 strain[J].Chemosphere, 2008, 74(1):26-32.
• [27] SCHANSKER G, TóTH S Z, STRASSER R J.Methylviologen and dibromothymoquinone treatments of pea leaves reveal the role of photosystem I in the Chl a fluorescence rise OJIP[J]. Biochimica et Biophysica Acta, 2005, 1706(3):250-261.
• [28] ESSEMINE J, QU M N, MI H L, et al. Response of chloroplast NAD(P)H dehydrogenase-mediated cyclic electron flow to a shortage or lack in ferredoxin-quinone oxidoreductase-dependent pathway in rice following short-term heat stress[J]. Frontiers in Plant Science,2016, 7:383.
• [29] NEELAM S, SUBRAMANYAM R. Alteration of photochemistry and protein degradation of photosystem II from Chlamydomonas reinhardtii under high salt grown cells[J]. Journal of Photochemistry and Photobiology B:Biology, 2013, 124:63-70.
• [30] PERREAULT F, AIT ALI N, SAISON C, et al.Dichromate effect on energy dissipation of photosystem II and photosystem I in Chlamydomonas reinhardtii[J].Journal of Photochemistry and Photobiology B, Biology,2009, 96(1):24-29.
• [31] SONG Y G, LIU B, WANG L F, et al. Damage to the oxygen-evolving complex by superoxide anion,hydrogen peroxide, and hydroxyl radical in photoinhibition of photosystem II[J]. Photosynthesis Research, 2006, 90(1):67-78.
• [32] STRASSER R J, SRIVASTAVA A, TSIMILLI-MICHAEL M. The fluorescence transient as a tool to characterize and screen photosynthetic samples[J]. Probing Photosynthesis:Mechanisms, Regulation and Adaptation,2000:445-483.