黄梁军,黄雷,杨娜,蔺凌云,姚嘉赟,陈静,沈锦玉,高迎莉,潘晓艺

• 1:江苏海洋大学海洋科学与水产学院
• 2:浙江省淡水水产研究所/农业农村部淡水渔业健康养殖重点实验室/浙江省鱼类健康与营养重点实验室/湖州市渔业环境与水产品质量安全重点实验室

摘要(Abstract)：

【目的】探究鰤鱼诺卡氏菌（Nocardia seriolae）弱毒株（LY20810 F110）免疫后大口黑鲈（Micropterus salmoides）头肾转录组的动态响应机制，为阐明该疫苗的免疫保护机制提供理论依据。【方法】将大口黑鲈分为免疫组（腹腔注射1×108 CFU/mL鰤鱼诺卡氏菌弱毒株）和对照组（注射生理盐水）。免疫7 d和28 d采集头肾组织进行RNA-seq测序，利用DESeq2筛选差异表达基因（DEGs），并对DEGs进行KEGG通路富集分析。免疫28 d后以强毒株攻毒，评估组织病理损伤并计算相对免疫保护率（RPS）。【结果】鰤鱼诺卡氏菌弱毒株免疫组的RPS达75%，攻毒后脏器无肉芽肿病变等组织损伤。转录组分析显示，免疫7 d和28 d分别鉴定出7 606和3 225个DEGs。KEGG富集表明免疫组显著上调先天免疫（FcεRI信号、补体C3）与适应性免疫（B细胞受体信号、NF-κB）相关通路，并激活IGH、CD22、PIGR等免疫相关基因的表达；同时下调促炎通路（IL-17信号、坏死性凋亡）。【结论】鰤鱼诺卡氏菌弱毒株LY20810 F110对大口黑鲈具有显著的免疫保护效果，其机制可能是通过激活先天免疫和适应性免疫相关通路以清除病原体，同时抑制过度的炎症反应，从而发挥免疫保护作用。

关键词(KeyWords)： 鰤鱼诺卡氏菌;减毒疫苗;大口黑鲈;免疫保护;转录组

Abstract:

Keywords:

基金项目(Foundation): 浙江省“三农九方”科技协作计划（2024SNJF051）;; 湖州市公益性应用研究（2021GZ28）;; 浙江省“尖兵领雁+X”研发攻关计划（2024C02005）;; 浙江省科研院所专项（2025YSZX02）

作者(Author): 黄梁军,黄雷,杨娜,蔺凌云,姚嘉赟,陈静,沈锦玉,高迎莉,潘晓艺

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