王海贞,王辉,李瑞伟,梁国栋

• 1:广东海洋大学水产学院
• 2:茂南三高罗非鱼良种繁殖基地

摘要(Abstract)：

采用中心组合设计和响应曲面法研究温度(12~34℃)和盐度(0~26)对吉富品系尼罗罗非鱼(Oreochromis niloticus)幼鱼(体长4.20±0.10 cm,体质量2.42±0.15 g)肠道超氧化物歧化酶(Superoxide Dismutase,SOD)、过氧化氢酶(Catalane,CAT)的联合效应。结果表明:1)温度对吉富罗非鱼幼鱼肠道SOD和CAT活力的线性效应和累积效应均极显著(P<0.01);盐度对吉富罗非鱼幼鱼肠道SOD活力线性效应显著(P<0.05),累积效应极显著(P<0.01);盐度对吉富罗非鱼幼鱼肠道CAT活力线性效应不显著(P>0.05),累积效应极显著(P<0.01);温度和盐度对SOD活力的互作效应极显著(P<0.01),对CAT活力的互作效应不显著(P>0.05)。2)响应曲面法分析表明,随着温度和盐度的增大,两种抗氧化酶的活力均呈先减小后增大的趋势。3)建立两种抗氧化酶活力与温度、盐度间关系的模型方程(SOD:决定系数R2=0.992 9,预测决定系数R2Pred.=0.939 2,P<0.01;CAT:决定系数R2=0.948 5,预测决定系数R2Pred.=0.647 1,P<0.01),两模型方程可用于预测吉富罗非鱼幼鱼肠道SOD和CAT的活力。4)优化结果显示,温度为24.15℃,盐度为11.75时,SOD活力最小值为9.80 U/mg,CAT活力最小值为9.28 U/mg,满意度函数值高达0.961。

关键词(KeyWords)： 吉富罗非鱼;温度;盐度;抗氧化酶;响应曲面法

Abstract:

Keywords:

基金项目(Foundation): 广东省海洋渔业科技推广专项项目[A2010002-010(b)];; 农业部公益性行业科研专项[200903046-02];; 广东省教育部产学研结合项目科技创新平台建设专项[2011B01048]

作者(Author): 王海贞,王辉,李瑞伟,梁国栋

参考文献(References)：

• [1]方允中,郑荣梁.自由基生物学的理论与应用[M].北京:科学出版社,2002:103-105.
• [2]Martinez-A’lvarez R M,Hidalgo M C,Domezain A,et al.Physiolo-gical changes of sturgeon Acipenser naccarii caused by increasingenvironmental salinity[J].Journal of experimental biology,2002,205(23):3699-3706.
• [3]周进,宋晓玲,黄倢,等.A3a肽聚糖对牙鲆不同组织中超氧化物歧化酶及磷酸酶活性的影响[J].中国水产科学,2004,11(4):296-301.
• [4]Morales A E,Pe’rez-Jime’nez A,Hidalgo M C,et al.Oxidative stressand antioxidant defenses after prolonged starvation in Dentexdentexliver[J].Comparative biochemistry and physiology,part C,2004,139(1-3):153-161.
• [5]Ross S W,Dalton D A,Kramer S,et al.Physiological(Antioxidant)responses of estuarine fishes to variability in dissolved oxygen[J].Comparative biochemistry and physiology,part C,2001,130(3):289-303.
• [6]杨丽华,方展强,郑文彪,等.镉对鲫鱼鳃和肝脏超氧化物歧化酶活性的影响[J].安全与环境学报,2003,3(3):13-16.
• [7]赵峰,庄平,章龙珍,等.施氏鲟不同组织抗氧化酶对水体盐度升高的响应[J].海洋水产研究,2008,29(5):65-69.
• [8]强俊,王辉,李瑞伟,等.盐度对奥尼罗非鱼仔、稚鱼生长、存活及其消化酶活力的影响[J].南方水产,2009,5(5):8-14.
• [9]王辉,强俊,李瑞伟.温度对奥尼罗非鱼仔稚鱼生长、饲料利用和消化酶活力的影响[J].广东海洋大学学报,2008,28(6):14-19.
• [10]Montgomery D C.Design and analysis of experiments[M].6th ed..New York:John Wiley&Sons,Inc.,2005:405-439.
• [11]Martinez-A’lvarez R M,Morales A E,Sanz A.Antioxidant defensesin fish:Biotic and abiotic factors[J].Reviews in fish biology andfisheries,2005,15(1-2):75-88.
• [12]李大鹏,刘松岩,谢从新,等.水温对中华鲟血清活性氧含量及抗氧化防御系统的影响[J].水生生物学报,2008,32(3):327-332.
• [13]Hochachka P W,Somero G N.Biochemical adaptation[M].London:Oxford university press,2002.
• [14]Lushchak V I,Bagnyukova T V.Temperature increase results inoxidative stress in goldfish tissues.1.Indices of oxidative stress[J].Comparative biochemistry and physiology,part C,2006,143(1):30-35.
• [15]Halliwell B,Gutteridge J M C.Free Radicals in Biology andMedicine[M].New York:Oxford university press,1999.
• [16]孔祥会,王桂忠,李少菁.低温驯化锯缘青蟹抗氧化防护、ATPase及膜脂肪酸组成变化[J].水生生物学报,2007,31(1):59-66.
• [17]Sies H.Strategies of antioxidant defense[J].European Journal ofBiochemistry,1993,215(2):213-219.
• [18]Parihar M S,Dubey A K.Lipid peroxidation and ascorbic acid statusin respiratory organs of male and female freshwater catfishHeteropneustes fossilis exposed to temperature increase[J].Comparative biochemistry and physiology,part C,1995,112(3):309-313.
• [19]李效宇,刘永定,宋立荣,等.鲤肝细胞抗氧化系统对微囊藻毒素毒性的反应[J].水生生物学报,2003,27(5):472-475.
• [20]Demple B.Radical ideas:genetic responses to oxidative stress[J].Clinical and experimental pharmacology and physiology,1999,26(1):64-78.
• [21]柳旭东,王际英,张利民,等.影响水产动物Na+-K+-ATPase活力的水环境因素[J].水产科学,2009,28(3):171-175.
• [22]Gaumet F,Boeuf G,Sévère A,et al.Effects of salinity of the ionicbalance and growth of juvenile turbot[J].Journal of fish biology,1995,47(5):865-867.
• [23]潘鲁青,唐贤明,刘泓宇.盐度对褐牙鲆(Paralich thysolivaceus)幼鱼血浆渗透压和鳃丝Na+-K+-ATPase活力的影响[J].海洋与湖沼,2006,37(1):1-6.
• [24]杨健,陈刚,黄建胜,等.温度和盐度对军槽鱼幼鱼生长与抗氧化酶活性的影响[J].广东海洋大学学报,2007,27(4):25-29.
• [25]Lotan R.Oxygen consumption in the gills of Tilapia aurea(Steindachner)(Pisces,Cichlidae)in various saline conditions[J].Israel journal of zoology,1966,15(2):33-37.
• [26]Palace V P,Dick TA,Brown S B,et al.Oxidative stress in lakesturgeon(Acipenser fulvescens)orally exposed to 2,3,7,8,-tetra-chlorodibenzofuran[J].Aquatic toxicology,1996,35(5):79-92.
• [27]Abele D,Tesch C,Wencke P.How oxidative stress parameters relateto thermal tolerance in the Antarctic bivalve Yoldia eightsi[J].Antarctic science,2001,13(2):111-118.
• [28]Mittler,R.Oxidative stress,antioxidants and stress tolerance[J].Trends in plant science,2002,7(9):405-410.