张燕飞,庞欢瑛,吴灶和,简纪常,鲁义善

• 1:广东海洋大学水产学院
• 2:广东省水产经济动物病原生物学及流行病学重点实验室
• 3:广东省水产经济动物病害控制重点实验室
• 4:仲恺农业工程学院

摘要(Abstract)：

双组分调控系统(Two-component Regulatory System)在致病菌的生长及毒力调控中起重要作用。克隆溶藻弧菌(Vibrio alginolyticus)HY9901株组氨酸激酶PhoR和反应调控因子PhoB的全长基因,并对其进行生物信息学分析。序列分析结果显示,pho R(Gen Bank登录号:KJ958404)全长1 299 bp,共编码432个氨基酸;pho B(Gen Bank登录号:KJ863646)全长690 bp,编码229个氨基酸。构建PhoR/PhoB的系统进化树,结果显示,溶藻弧菌PhoR/PhoB与副溶血弧菌(Vibrio parahaemolyticus)、哈氏弧菌(Vibrio harveyi)有较近亲缘关系。利用SWISS-MODEL软件,对PhoR/PhoB中两个相对保守的功能域HATPase_c和REC进行同源建模,发现HATPase_c具有1个ATP结合位点,REC具有4个天冬氨酸活性位点。

关键词(KeyWords)： 溶藻弧菌;双组分调控系统;PhoR/PhoB;基因克隆;生物信息学

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑项项目(2012BAD17B02、2012BAD17B03);; 广东高校国际合作创新平台项目(2013gjhz0008)

作者(Author): 张燕飞,庞欢瑛,吴灶和,简纪常,鲁义善

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