林听听,来琦芳,陆建学,么宗利,李子牛,王慧,周凯

• 1:中国水产科学研究院东海水产研究所中国水产科学研究院盐碱地渔业工程技术研究中心

摘要(Abstract)：

研究了盐度23下青蛤(Cyclina sinensis)鳃、斧足、外套膜和内脏团中Na+/K+-ATPase的活性差异,并以活性高的鳃组织为对象,对青蛤实施了低盐(23→16)和高盐(23→36)突变的胁迫,监测了鳃组织中Na+/K+-ATPase活性的动态变化。结果表明,盐度23下青蛤鳃组织中Na+/K+-ATPase的活性显著高于其他组织;在盐度突降胁迫下,鳃组织中Na+/K+-ATPase的活性于前16 d逐渐升高,第17d起显著升高(p<0.05),第18 d达到峰值,于第20 d回落,但仍高于起始水平;在盐度骤升胁迫下,鳃组织中Na+/K+-ATPase的活性于前13d逐渐降低,第14 d起显著降低(p<0.05),于第15 d达到最低值,继而回升至平稳水平,但仍低于起始水平。青蛤不同盐度胁迫下其鳃组织中Na+/K+-ATPase活性的响应方式不同,低盐时活性增加,而高盐时活性下降;此外,青蛤在盐度胁迫下对自身渗透压的调节则需较长时间。

关键词(KeyWords)： 青蛤;鳃;盐度突变;Na+/K+-ATPase

Abstract:

Keywords:

基金项目(Foundation): 上海市科委技术标准专项(10DZ0503100)

作者(Author): 林听听,来琦芳,陆建学,么宗利,李子牛,王慧,周凯

参考文献(References)：

• [1]Satyavathi C,Rao Y P.Inhibition of Na+/K+-ATPase in Penaeusindicus postlarvae by lead[J].Comparative Biochemistry andPhysiology Part C,2000,127(1):11 22.
• [2]Towel D W.Role of Na+/K+-ATPase in ionic regulation by marineand estuarine animals[J].Marine biology letters,1981,2(1):107 122.
• [3]Chen J C,Nan F H.Effect of ambient ammonia on ammonia-Nexcretion and ATPase activity of Penaeus chinensis[J].AquaticToxicology,1992,23(2):1 l0.
• [4]唐贤明,潘鲁青,田景波.褐牙鲆鳃丝Na+/K+-ATPase性质的研究[J].中国海洋大学学报,2004,36(6):969972.
• [5]徐力文,苏友禄,刘广锋,等.急性盐度胁迫下军曹鱼稚鱼应激反应的血清学指标[J].华南农业学学学报,2007,28(2):9194.
• [6]Handeland S O,Stefansson S O.Effects of salinity acclimation onpre-smolt growth,smolting and post-smolt performance in off-seasonAtlantic salmon smolts(Salmo salar L.)[J].Aquaculture,2002,209(1-4):125 137.
• [7]房文红,王慧,来琦芳,等.斑节对虾鳃Na+/K+-ATPase的活性[J].上海水产大学学报,2001,10(2):140144.
• [8]潘鲁青,刘志,姜令绪.盐度、pH变化对凡纳滨对虾鳃丝Na+/K+-ATPase活力的影响[J].中国海洋大学学报,2004,34(5):787 790.
• [9]潘鲁青,栾治华.盐度对日本囊对虾仔虾生长发育和Na+/K+-ATPase活力的影响[J].水生生物学报,2005,29(6):699 703.
• [10]王顺昌,于敏.中华绒螯蟹在不同盐度下鳃Na+/K+-ATPase和ALP活性的变化[J].安徽技术师范学院学报,2003,17(2):117120.
• [11]王如才,王昭萍,张建中.海水贝类养殖学[M].青岛:青岛海洋大学出版社,1993,p.373376.
• [12]王丹丽,徐善良,优仲杰,等.温度和盐度对青蛤孵化及幼虫、稚贝存活与生长变态的影响[J].水生生物学报,2005,29(5):495 501.
• [13]曹华.沿海滩涂青蛤死亡原因及对策[J].中国水产,2004,(4):57–58.
• [14]孙国铭,万夕和,刘培庭,等.通州海区滩涂青蛤死亡原因的初步分析[J].水产养殖,2004,25(2):26–27.
• [15]Wheat M G,Henry R P.Branchial and antennal glandNa+/K+-dependent ATPase and carbonic anhydrase activity duringsalinity acclimation of the euryhaline crayfish Pacifastacusleniusculus[J].Journal of Experimental Biology,1987,133:73 86.
• [16]Henry R P,Kormanik G A.Carbonic anhydrase activity and calciumdepositin during the milt cycle of the blue crab Callinetes sapidus[J].Journal of Crustacean Biology,1985,5(2):234 241.
• [17]袁春营,崔青曼.盐度影响水产养殖动物生长发育的研究进展[J].水产科学,2004,23(5):41–42.
• [18]尤仲杰,徐善良,边平江.海水温度和盐度对泥蚶幼虫和稚贝生长及存活的影响[J].海洋学报,2001,23(6):108–113.
• [19]Allan G L,Maguire G B.Effects of pH and salinity on survival,growth and osmoregulation in Penaeus monodon Fabricius[J].Aquaculture,1992,107(1):33 47.
• [20]Mallatt J.Fish gill structural changes induced by toxicants and otherirritants:A statistical review[J].Canadian Journal of Fisheries andAquatic Sciences,1985,42:630 648.
• [21]苗晶晶,潘鲁青,刘志.铜、锌对褐牙鲆鳃丝Na+/K+-ATPase活力的影响[J].中国水产科学,2005,12(3):340343.
• [22]郭春雨,管越强,刘波兰.温度、pH和盐度对克氏原螯虾鳃Na+-K+-ATPase活性的影响[J].动物学杂志,2007,42(6):96–102.
• [23]Lucu C,Devescovi M.Osmoregulation and branchialNa+,K+-ATPase in the lobster Homarus gammarus acclimated todilute seawater[J].Journal of experimental marine biology andecology,1999,234(2):291–304.
• [24]Ferraris R P,Parado-Estepa F D,Ladfa J M,et a1.Effect of salinityon the osmotic,chloride,total protein and calcium concentrations inthe hemolymph of the prawn Penaeus monodon[J].ComparativeBiochemistry and Physiology Part B,1986,83:701 708.
• [25]Parado-Estepa F D,Ferraris R P,Ladja J M,et al.Responses ofintermolt Penaeus indicus to large fluctuations in environmentalsalinity[J].Aquaculture,1987,64:175 184.
• [26]Neufeld G J,Holliday C W,Pritchard J B.Salinity adaptation of GillNa+/K+-ATPase in the blue Crab,Callinectes sapidus[J].Journal ofExperimental Zoology,1980,211:215 224.