鰤鱼诺卡氏菌与绛红色诺卡氏菌的融合菌研究Study on Protoplasts fusion of Nocardia seriolae and N.purpurea
陈建林,严聪羚,张红莲,苏泽杰,夏立群
摘要(Abstract)：
鰤鱼诺卡氏菌(Nocardia seriolae)为鱼类诺卡氏菌病的主要病原,绛红色诺卡氏菌(N.purpurea)是分离自土壤中一种无毒诺卡氏菌。以鰤鱼诺卡氏菌和绛红色诺卡氏菌为亲本进行原生质体融合,获取融合菌。经生长特征观察、革兰染色、16SrRNA测序分析,对融合菌进行初步鉴定,并通过斑马鱼模型评估融合菌毒力检测和免疫保护效果。结果表明,融合菌的菌落形态与亲本菌株不同,为诺卡氏菌属革兰阳性菌,融合菌的毒力较鰤鱼诺卡氏菌野生株降低70%,免疫保护率约为50%。
关键词(KeyWords)： 鰤鱼诺卡氏菌;绛红色诺卡氏菌;原生质体融合;毒力;免疫保护
基金项目(Foundation): 广东省科技发展专项资金项目(2016A050502061);; 广东省自然科学基金(2014A030313602);; 深圳大鹏新区产业发展专项资金项目(KY20160207);; 广东省攀登计划项目(pdjh2016b0234)
作者(Author): 陈建林,严聪羚,张红莲,苏泽杰,夏立群
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