运用ITS基因分析大鹏半岛海域石珊瑚系统发育关系Phylogenetic Relationships in Scleractinian from Water of Dapeng Peninsula Based on ITS Genes
谢子强,廖宝林,肖宝华,杨小东,李晓洁,陈园媛
摘要(Abstract):
通过ITS基因特异扩增测序,对大鹏半岛海域46种石珊瑚ITS基因片段序列进行了比较分析。结果表明,该片段序列长度在674~806碱基对(bp)之间,A、T含量在40%~55.5%之间,大部分物种的序列碱基A、T含量小于G、C含量。46种造礁珊瑚的平均遗传距离为0.261。采用邻位连接(NJ)和最小进化(ME)法分别构建15种复合型珊瑚的5.8S r DNA系统发育树和28种坚实型珊瑚的ITS2系统发育树,结果显示15种复合型珊瑚的5.8S r RNA序列系统进化聚类结果较符合传统形态学分类结果,而28种坚实型珊瑚的ITS2序列系统进化聚类结果与传统形态学存在较大的差异,提示石珊瑚表型的变异性可能对传统分类存在影响。
关键词(KeyWords): 石珊瑚;ITS;分子系统学
基金项目(Foundation): 广东省公益研究与能力建设专项(K15216);; 广东省海洋渔业科技推广专项(A201308E02);; 大鹏新区产业发展专项资金(DPKJ201500080);大鹏新区产业发展专项资金(KY20160107)
作者(Author): 谢子强,廖宝林,肖宝华,杨小东,李晓洁,陈园媛
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