微囊藻毒素对凡纳滨对虾免疫相关酶活力的影响Effects of Microcystin on Activities of Immune Enzymes in the White Shrimp Litopenaeus Vannamei
陈妍妍,李长玲,黄翔鹄
摘要(Abstract):
通过毒性注射实验,探究微囊藻毒素(MC-LR)对凡纳滨对虾(Litopenaeus Vannamei)肝脏免疫相关酶活力的影响。结果表明,注射MC-LR后,凡纳滨对虾肝胰脏组织的超氧化歧化酶、谷胱甘肽过氧化酶、过氧化物酶、酸性磷酸酶、碱性磷酸酶和溶菌酶的酶活力呈先增大后减小的变化趋势。超氧化歧化酶、谷胱甘肽过氧化酶和过氧化物酶活力在对虾注射MC-LR 4 h后达到峰值,比对照组分别增加了15.62%、18.76%和32.86%,随后酶活力逐渐减小;而酸性磷酸酶和碱性磷酸酶酶活力在对虾注射MC-LR 2 h后即显著增加,比对照组分别增加了25.02%和37.99%,随后酶活力逐渐减小;溶菌酶在对虾注射MC-LR后8 h达到最大值,比对照组增加了33.57%,随后酶活力逐渐减小。MC-LR能显著影响凡纳滨对虾的免疫相关酶活力(P<0.05),引起凡纳滨对虾的应激反应和抑制它们的免疫相关酶活力。
关键词(KeyWords): 微囊藻毒素(MC-LR);凡纳滨对虾;免疫酶
基金项目(Foundation): 公益性行业(农业)科研专项(201203083);; 广东省专业镇中小微企业服务平台建设项目(2012B040500025);; 广东省海洋渔业科技推广专项(A201308B10)
作者(Author): 陈妍妍,李长玲,黄翔鹄
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