杨文聪,刘亚月,杨静明,黎钊坪,梁金月,聂影影,马小翔,张翼

• 1:广东海洋大学深圳研究院
• 2:广东海洋大学食品科技学院//广东省水产品加工与安全重点实验室//广东省海洋生物制品工程实验室//广东省海洋食品工程技术研究中心//水产品深加工广东普通高等学校重点实验室//广东海洋大学海洋药物研究所

摘要(Abstract)：

【目的】通过化学诱导方法从一株海洋真菌Hypocrea lixii中获得多样化的活性小分子化合物。【方法】对菌株在盐酸普鲁卡因的化学诱导作用下进行固体发酵,代谢产物采用硅胶减压柱层析、SephadexLH-20以及pHPLC等手段分离纯化,对化合物运用NMR、MS等波谱学技术并比对文献进行结构鉴定,采用Ellman比色法及DPPH自由基清除法对化合物进行初步抗乙酰胆碱酯酶(ACh E)和抗氧化活性测试。【结果】从海洋真菌Hypocrea lixii在盐酸普鲁卡因诱导下的发酵提取物中分离得到4个异黄酮类化合物,包括6'-O-crotonylgenistin(1)、Genistein(2)、Daidzein(3)和Genistin(4),以及对氨基苯甲酸甲酯(5)、对羟基苯丙酸(6)和胞嘧啶(7),其中化合物1和6为该种中首次报道,化合物5为生物转化来源首次报道,化合物2～4可能源自培养基。在100μmol/L剂量浓度下,化合物1～4对ACh E的抑制以及DPPH自由基的清除活性均较弱,化合物5和6显示了相对强的ACh E抑制活性,抑制率分别为40%和38%。【结论】盐酸普鲁卡因对该菌次级代谢产生了显著的影响,为深入研究该菌株化学诱导次级代谢产物提供基础。

关键词(KeyWords)： 海洋真菌;Hypocrea lixii;化学诱导;次生代谢产物

Abstract:

Keywords:

基金项目(Foundation): 广东省扬帆计划引进紧缺拔尖人才项目(201433009);; 国家自然科学基金(21807015);; 广东省自然科学基金(2018A030307046);; 深圳市科创委基础研究自由探索项目(JCYJ20170306165013264);; 广东省应用科技研发重大专项(2016B020235001);; 广东省促进经济发展专项资金(海洋经济发展用途)项目(粤自然资源合[2019] 015号);; 深圳市大鹏新区产业发展资金(KY20180203及PT201901-05);; 湛江市海洋渔业局项目(A18018)

作者(Author): 杨文聪,刘亚月,杨静明,黎钊坪,梁金月,聂影影,马小翔,张翼

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