张卫,谭北平,庞奥博,邓君明,杨奇慧,张海涛

• 1:广东海洋大学水产学院
• 2:农业部南方水产与畜禽饲料重点实验室

摘要(Abstract)：

【目的】筛选珍珠龙胆石斑鱼（Epinephelusfuscoguttatus♀×Epinepheluslanceolatus♂）豆粕诱导型肠炎（SBMIE）肠道组织的潜在生物标志物。【方法】在基础饲料中用豆粕替代质量分数0%、20%和40%的鱼粉蛋白（分别记为FM、SBM20和SBM40组），配制3种等氮（粗蛋白质量分数50%）等脂（粗脂肪质量分数10%）饲料，饲喂初始体质量约9 g的珍珠龙胆石斑鱼10周，分析石斑鱼生长性能、肠道组织结构、炎性指标和肠炎表征；通过非靶向代谢组学技术，筛选石斑鱼SBMIE肠道组织潜在生物标志物。【结果】实验水平豆粕替代鱼粉对珍珠龙胆石斑鱼的增重率、特定生长率和肝体指数有显著负面影响（P <0.05）；对存活率无显著影响（P> 0.05）；免疫指标和H&E组织切片显示，肠炎症状随SBM增多逐渐加重。SBM20和SBM40组中分别发现17和18种SBMIE潜在生物标志物，其中有13种核心生物标志物为两组共有，包括染料木素、大豆黄酮、染料木素-4’-O葡萄糖醛酸酯、鸡黄豆素、柠檬苦素、吲哚-3-丙烯酰甘氨酸、白三烯B4二甲胺、维生素C、黄芩苷、葡萄糖二酸、大豆皂苷Ⅰ、小肽（Leu-Gly-Pro）和戊基-4-羟脯氨酸。所有标志物中，除柠檬苦素、吲哚-3-丙烯酰甘氨酸、Leu-Gly-Pro、戊基-4-羟脯氨酸、癸二酸、磷酸肌酸、N,N-二甲基甲氧基噻吩、N-甲基蒽酰胺和2-氨基-3,4-二甲基咪唑（4,5-F）喹啉与FM组比强度显著降低外（P <0.05），其余强度均显著升高（P <0.05）。大部分标志物间有显著正相关或负相关作用（P <0.05）。【结论】在SBMIE状态下，珍珠龙胆石斑鱼肠道组织中存在特征性的潜在核心生物标志物。

关键词(KeyWords)： 珍珠龙胆石斑鱼;豆粕;代谢组学;肠炎;生物标志物

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划(2019YFD0900200);; 财政部和农业农村部：国家现代农业产业技术体系项目(CARS-47)

作者(Author): 张卫,谭北平,庞奥博,邓君明,杨奇慧,张海涛

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