陈贺,吴灶和,王蓓,黄郁葱,鲁义善,简纪常

• 1:广东海洋大学水产学院
• 2:广东省水产经济动物病原生物学及流行病学重点试验室暨广东省高等学校水产经济动物病害控制重点实验室
• 3:仲恺农业工程学院

摘要(Abstract)：

研究无乳链球菌(Streptococcus agalactiae)ZQGY08的培养与生长特性,探讨脑心浸液(Brian Heart Infusion,BHI)培养基的营养因子、初始pH值、溶氧、温度等因素对无乳链球菌生长的影响,并采用正交实验法对无乳链球菌培养基营养因子添加量进行优化。结果表明,无乳链球菌生长的最适初始pH为7～8,培养温度为28～37℃,振荡转速为180～200 r/min。在BHI培养基中添加20 mg/mL的酵母粉和2 mg/mL的葡萄糖即可在对数期获得最大细菌量。

关键词(KeyWords)： 无乳链球菌;培养基;培养条件;优化

Abstract:

Keywords:

基金项目(Foundation): 广东省科技计划项目“罗非鱼无乳链球菌病免疫防治技术的研究”(2010B020309009)

作者(Author): 陈贺,吴灶和,王蓓,黄郁葱,鲁义善,简纪常

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