刘凤坤,赵吉臣,许敬轩,郑雅之,鲁慧杰,孙育平,阮灼豪,郭慧,杨俊贤,黄文

• 1:广东海洋大学水产学院
• 2:广东省农业科学院动物科学研究所/广东省畜禽育种与营养研究重点实验室/农业农村部华南动物营养与饲料重点实验室/广东省农业科学院水产研究中心
• 3:珠海市金湾区平沙镇社会事务服务中心

摘要(Abstract)：

【目的】分离鉴定一株耐盐好氧反硝化细菌，以开发高效脱氮菌种，加强海水及咸淡水养殖尾水脱氮技术研究。【方法】采集花鲈（Lateolabrax maculatus）池塘原位底泥，采用稀释涂布法初步分离一株脱氮功能最佳的菌株HL4，通过16s rDNA测序以及生理生化实验鉴定菌株，通过设置不同碳源、碳氮质量比（m_C/m_N）、pH、温度、盐度等条件探明其最适培养条件及脱氮性能。通过攻毒试验[以健康斑马鱼（Danio rerio）为受试生物]，检验菌株的环境安全性。【结果】经鉴定，菌株HL4为黏质沙雷氏菌（Serratia marcescens），命名为S.marcescens HL4。以葡萄糖或琥珀酸钠为碳源，菌株HL4在m_C/m_N=20、pH=6.0～7.0、温度30℃的条件下具有较强脱氮能力，48 h TN去除率为81.83%～87.17%；该菌株在0～30的盐度环境下均可生长，在0～10盐度范围内72 h的总氮（TN）去除率为68.76%～76.59%，在20～30盐度范围内72 h的TN去除率为23.84%～53.94%；在NH_4~+-N质量浓度为0～100 mg·L~(-1)时，72 h NH_4~+-N去除率皆在90%以上，在NH_4~+-N质量浓度为500 mg·L~(-1)时，72 h NH_4~+-N去除率高达73.52%。健康斑马鱼在含1×10~6CFU·mL~(-1)菌株HL4的水体中15 d后，成活率100%。【结论】菌株HL4生物及环境安全性较高，在处理中高浓度铵废水以及治理咸淡水、低盐度海水养殖尾水等领域具有潜在的应用价值。

关键词(KeyWords)： 黏质沙雷氏菌;好氧反硝化;耐盐;生物安全性;环境因子;脱氮能力

Abstract:

Keywords:

基金项目(Foundation): 广东省农业科学院人才项目（R2020YJ-QG001,R2022PY-QY008）;广东省农业科学院协同创新中心课题（202137,202138,XT202231）;; 珠海市斗门区白蕉镇政府服务项目（ZHWZ2021-403FW,ZHWZ2021-408FW）;; 乡村振兴战略专项资金（农业科技能力提升）项目（2023工作站12）;;

作者(Author): 刘凤坤,赵吉臣,许敬轩,郑雅之,鲁慧杰,孙育平,阮灼豪,郭慧,杨俊贤,黄文

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