张顺,廖健,郭昱嵩,王中铎,刘楚吾

• 1:广东海洋大学南海水产经济动物增养殖广东普通高校重点实验室

摘要(Abstract)：

于我国雷州半岛红树林海区捕获隶属于弹涂鱼属(Periophthalmus)、大弹涂鱼属(Boleophthalmus)与青弹涂鱼属(Scartelaos)的4种弹涂鱼,克隆4种弹涂鱼23个个体cox1基因序列,通过分析4种弹涂鱼与Gen Bank中12种弹涂鱼cox1基因序列的变异特征,探讨4种弹涂鱼与后者的种间亲缘关系。结果表明,16种弹涂鱼的82个体的cox1基因序列共检测到单倍型44个,核苷酸多态位点212个(占35.4%),种间平均核苷酸差异数为4(占2.01%);基于最大似然法(ML)和邻接法(NJ)构建的系统发育树显示,各弹涂鱼属多形成一独立分支,亲缘关系明显,齿弹涂鱼属(Periophthalmodon)镶嵌于弹涂鱼属内,大弹涂鱼属与青弹涂鱼属形成并列分支,亲缘关系较近。依化石记录标定的松散分子钟估算物种分歧时间,结果表明,各弹涂鱼属出现明显分化大约发生于渐新世末期至中新世早期(23.61~15.65 Ma)。

关键词(KeyWords)： 弹涂鱼;线粒体DNA;cox1基因;系统进化

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31201996);; 广东省高等学校优秀青年教师培养计划项目(Yq2013089号);; 广东海洋大学“海之帆—起航计划”大学生科技创新项目(hzfqhjhkjfm2015b11)

作者(Author): 张顺,廖健,郭昱嵩,王中铎,刘楚吾

参考文献(References)：

• [1]MRUDY E O.A taxonomic revision and cladistic analysis of the Oxudercine Gobies(Gobiidae:Oxudercinae)[J].Records of the Australian Museum,1989,11:1-93.DOI:10.3853/j.0812-7387.11.1989.93.
• [2]伍汉霖,钟俊生.中国动物志(硬骨鱼纲):鲈形目5(虾虎鱼亚目)[M].北京:科学出版社,2008:1-718.
• [3]ANSARI A A,TRIVEDI S,SAGGU S,et al.Mudskipper:A biological indicator for environmental monitoring and assessment of coastal waters[J].Journal of Entomology and Zoology Studies,2014,2(6):22-33.
• [4]张顺,廖健,柏琴,等.COⅠ条形码辅助分析雷州半岛红树林区鱼类的物种多样性[J].海洋与湖沼,2016,47(3):663-672.
• [5]HEBERT P D N,CYWINSKA A,Ball S L,et al.Biological identifications through DNA barcodes[J].Proceedings of the Royal Society B:Biological Sciences,2003,270(1512):313-321.DOI:10.1098/rspb.2002.2218.
• [6]WARD R D,ZEMLAK T S,INNES B H et al.DNA barcoding Australia’s fish species[J].Philosophical Transactions of the Royal Society B:Biological Sciences,2005,360(1462):1847-1857.DOI:10.1098/rstb.2005.1716.
• [7]JEON H B,CHOI S H,SUK H Y.Exploring the utility of partial cytochrome c oxidase subunit 1 for DNA barcoding of gobies[J].Animal Systematics,Evolution and Diversity,2012,28(4):269-278.
• [8]THACKER C E,ROJE D M.Phylogeny of Gobiidae and identification of gobiid lineages[J].Systematics and Biodiversity,2011,9(4):329–347.DOI:10.1080/14772000.2011.629011.
• [9]廖健,张顺,龙水生,等.5种虾虎鱼类线粒体COⅠ基因序列变异及系统进化[J].广东海洋大学学报,2016,36(1):7-12.
• [10]廖健,贺亮,张顺,等.基于cox1和D-loop区序列雷州半岛红树林海区2种野生弹涂鱼的遗传变异[J].广东海洋大学学报,2016,36(4):6-11.
• [11]YOU X X,BIAN C,ZAN Q J,et al.Mudskipper genomes provide insights into the terrestrial adaptation of amphibious fishes[J/OL].Nature Communications,2014,5:5594.doi:10.1038/ncomms6594.
• [12]AGORRETA A,SAN MAURO D,SCHLIEWEN U,et al.Molecular phylogenetics of Gobioidei and phylogenetic placement of European gobies[J].Molecular Phylogenetics and Evolution,2013,69(3):619–633.
• [13]GHANBARDI M,ESMAEILI H R,GHOLAMI Z,et al.Molecular phylogeny of three mudskippers(Gobiidae)from the Persian Gulf and Gulf of Oman(Makran)[J].Journal of Applied Ichthyology,2016,32(3):416–420.
• [14]陈卉,POLGAR G,殷维,等.西北太平洋海岸带大弹涂鱼复合体的隐存种与进化历史[J].水生生物学报,2014,38(1):75-86.
• [15]POLGAR G,ZANE L,BABBUCCI M,et al.Phylogeography and demographic history of two widespread Indo-Pacific mudskippers(Gobiidae:Periophthalmus)[J].Molecular Phylogenetics and Evolution,2014,73:161–176.
• [16]伍汉霖,邵广昭,赖春福,等.拉汉世界鱼类系统名典[M].基隆:水产出版社,2012:10-550.
• [17]萨姆布鲁克J,拉塞尔D W.分子克隆实验指南[M].黄培堂,王嘉玺,朱厚础,译.3版.北京:化学工业出版社,2008:693.
• [18]KUMAR S,STECHER G,TAMURA K.MEGA7:Molecular evolutionary genetics analysis version 7.0 for bigger datasets[J].Molecular Biology and Evolution,2016,33(7):1870-1874.
• [19]LIBRADO P,ROZAS J.Dna SP v5:a soft ware for comprehensive analysis of DNA polymorphism data[J].Bioinformatics,2009,25(11):1451-1452.DOI:10.1093/bioinformatics/btp187.
• [20]GUINDON S,GASCUEL O.A simple,fast,and accurate algorithm to estimate large phylogenies by maximum likelihood[J].Systematic Biologists,2003,52(5):696–704.DOI:10.1080/10635150390235520
• [21]MILLER P J.The osteology and adaptive features of Rhyacichthys aspro(Teleostei:Gobioidei)and the classification of gobioid fishes[J].Journal of Zoology,1973,171(3):397–434.
• [22]YAO Y G,KONG Q P,SALAS A,et al.Pseudo-mitochondrial genome haunts disease studies[J].Journal of Medical Genetics,2008,45(12):769–772.
• [23]SHACKELTON L A,PARRISH C R,HOLMES E C,Evolutionary basis of codon usage and nucleotide composition bias in vertebrate DNA viruses[J].Journal of Molecular Evolution,2006,62(5):551–563.
• [24]MOOERS,A?,HOLMES E C,The evolution of base composition and phylogenetic inference[J].Trends in Ecology&Evolution,2000,15(9):365-369.
• [25]BERMINGHAM E,AVISE J C.Molecular zoogeography of freshwater fishes in the southeastern United States[J].Genetics,1986,113(4):939-965.
• [26]ZHANG J,TANIGUCHI T,TAKITA T,et al.A study on the epidermal structure of Periophthalmodon and Periophthalmus mudskippers with reference to their terrestrial adaptation[J].Ichthyological Research,2003,50(4):310-317.
• [27]王中铎,郭昱嵩,陈荣玲,等.南海常见硬骨鱼类CO I条码序列[J].海洋与湖沼,2009,40(5):608-614.
• [28]BAJPAI S,KAPUR V V.Oldest known gobiids from Vastan Lignite Mine(early Eocene),Surat District,Gujarat[J].Current Science,2004,87(4):433-435.
• [29]COLBORN J,CRABTREE R E,SHAKLEE J B,et al.The evolutionary enigma of bonefishes(Albula spp.):cryptic species and ancient separations in a globally distributed shorefish[J].Evolution,2001,55(4):807–820.