稀杯盔形珊瑚转录组分析Transcriptome Analysis of Galaxea astreata
刘金豆,刘丽,黄元佳
摘要(Abstract)：
以高通量测序技术Illumina Hi SeqTM2000对稀杯盔形珊瑚(Galaxea astreata)进行转录组测序分析,共获得50 360 620条短序列(reads)。利用Trinity软件对所有reads从头组装后得到81 014条单基因簇(Unigenes),60 471条(74.58%)编码蛋白框(Coding Sequences,CDs)。与Nr(Non-redundant,非冗余)、COG(Cluster of Orthologous Groups of proteins,蛋白相邻类的聚簇)、KEGG(Kyoto encyclopedia of genes and genomes,京都基因与基因组百科全书)、Swissprot四大数据库比对共获得36 545条注释基因。其中与COG数据库比对获得14 491条注释基因,分为24个功能类别,其中参与一般功能预测类的Unigene数最多,有4 642条;KEGG分析比对获得16 021条注释基因,分成241类,包括代谢通路、钙离子信号通路、MAPK信号通路等,在所有通路中参与代谢途径的基因数最多,共有2 450条(15.29%);GO(Gene Ontology,基因本体)功能分类将Unigene分为47个类别。
关键词(KeyWords)： 转录组;稀杯盔形珊瑚;Unigene;信号通路
基金项目(Foundation): 国家海洋公益性行业科研专项(201105012);; 广东省自然科学基金项目(S2011010000269);; 广东省海洋渔业科技推广专项(A201308E02)
作者(Author): 刘金豆,刘丽,黄元佳
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