廖文钰,竺利波,刘明珠,余庆,玉洁莹,陈嘉,韦云依,黄吉利,黄琳,李鹏飞

• 1:广西科学院广西海洋科学院/广西水产生物技术与现代生态养殖重点实验室/广西渔业重大疫病防控与高效健康养殖产业技术工程研究中心
• 2:北部湾大学海洋学院
• 3:南宁学院食品与质量工程学院
• 4:北部湾海洋产业研究院/中国-东盟现代渔业产业技术转移示范中心

摘要(Abstract)：

【目的】探究肉桂醇提物在大口黑鲈（Micropterus salmoides）虹彩病毒（LMBV）感染中的抗病毒效果及作用机制，为开发抗LMBV药物提供参考。【方法】采用CCK-8试剂盒检测、结晶紫染色观察、实时荧光定量PCR(RT-q PCR）检测、病毒滴度[半数组织培养感染剂量（TCID_(50)）]测定等方法确定肉桂醇提物在胖头鱥肌肉细胞系（FHM）上的安全工作浓度，评估肉桂醇提物在细胞水平对LMBV的抗病毒效果，分析其抗LMBV机制。【结果】肉桂醇提物在≤12.5μg/m L的质量浓度范围内对FHM细胞无明显毒性。FHM细胞与LMBV以及质量浓度分别为12.5、6.25、3.125μg/m L肉桂醇提物共孵育后，细胞病变明显减少，结晶紫染色后可见活细胞数明显升高，MCP、ICP46基因的表达量显著降低（P<0.01），且抗病毒效果有剂量依赖性。12.5μg/m L肉桂醇提物与LMBV共孵育后接入FHM细胞，细胞中MCP、ICP46基因的相对表达量以及TCID_(50)极显著下降（P<0.01);FHM细胞与经肉桂醇提物处理的LMBV共孵育后，细胞中LMBV MCP和ICP46基因的相对表达量显著低于未用肉桂醇提物处理的LMBV感染组（P<0.01）；在LMBV感染FHM细胞后，接入肉桂醇提物的FHM细胞中MCP和ICP46基因的相对表达量显著下降（P<0.05）；在LMBV感染FHM细胞3 h后用肉桂醇提物处理8 h的FHM细胞中MCP和ICP46基因的相对表达量显著下调（P<0.05）。【结论】肉桂醇提物可降低LMBV的感染力，干扰LMBV侵染宿主细胞过程中的吸附、侵入及复制，有良好的抗LMBV作用，可作为环保、高效的抗LMBV渔用药物候选者。

关键词(KeyWords)： 大口黑鲈;虹彩病毒;肉桂;抗病毒作用;渔用药物

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（32373175）;; 广西自然科学基金（2023JJG130007,2022GXNSFBA035521,2022JJA130074）;; 国家重点研发计划（2022YFD2401200）;; 国家现代农业产业技术体系广西创新团队项目（nycytxgxcxtd-2021-08-02）

作者(Author): 廖文钰,竺利波,刘明珠,余庆,玉洁莹,陈嘉,韦云依,黄吉利,黄琳,李鹏飞

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