2种亚洲龙鱼的D-Loop序列结构分析Analysis on the D-loop Structure of Two Stock of Scleropages formosus
潘阳,白俊杰,叶星,劳海华,罗建仁,汪学杰
摘要(Abstract):
采用PCR技术对2种亚洲龙鱼的mtDNAD_Loop全序列进行扩增和测序,序列结构分析和序列同源性比对结果表明,2种亚洲龙鱼的mtDNAD_Loop在靠近5’端有3个终止相关序列TAS(Ⅰ、Ⅱ、Ⅲ),靠近D_Loop的3’端有4个保守区域CSB1、CSB2、CSB3、CSB-D。在终止相关序列和保守区域之间是连续重复区域。经DNASP4.0软件分析,全序列中检测出多态位点数(S)为26,其中有17个转换,核苷酸多样性(Pi)为0.013,平均核苷酸差异数(K)为17.333。
关键词(KeyWords): 亚洲龙鱼;D-Loop序列;序列结构
基金项目(Foundation): 中国水产科学研究院基金(2003_3_5)
作者(Author): 潘阳,白俊杰,叶星,劳海华,罗建仁,汪学杰
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