陈爽,梁健,张荣庆

• 1:蛋白质科学教育部重点实验室清华大学生命科学学院
• 2:浙江省应用酶学重点实验室
• 3:嘉兴大学生物化学科学与工程学院

摘要(Abstract)：

【目的】克隆合浦珠母贝(Pinctada fucata)丝氨酸蛋白酶抑制因子pfser1基因,探讨该基因的组织表达及其在天然免疫过程中的作用,以及与生物矿化过程的关系。【方法】通过RACE技术获得pfser1基因的全长,通过生物信息学分析其序列结构特征,利用实时荧光定量PCR方法检测pfser1基因在不同组织中的表达,检测健康合浦珠母贝在被大肠杆菌(Escherichia coli)MG1655刺激后和在贝壳损伤修复实验中pfser1基因表达量的变化。【结果】合浦珠母贝pfser1基因c DNA全长为1 240 bp,包含1 035 bp的开放阅读框(ORF),编码344个氨基酸,氨基酸序列的功能结构域含有丝氨酸蛋白酶抑制因子Serpin家族保守结构域。pfser1基因在合浦珠母贝各个组织中均有表达,在外套膜边缘膜中表达量最高;大肠杆菌MG1655刺激后,该基因表达量显著升高;在贝壳损伤修复过程中,pfser1基因表达先升高后受到抑制。【结论】pfser1基因所表达的蛋白参与了合浦珠母贝的天然免疫应答过程,并与生物矿化过程有一定关系。

关键词(KeyWords)： 合浦珠母贝;丝氨酸蛋白酶抑制因子;基因克隆;基因表达;天然免疫

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31872543,31572594)

作者(Author): 陈爽,梁健,张荣庆

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