方玲玲,王忠良,陈刚,汤保贵,张健东,黄建盛,周晖

• 1:广东海洋大学水产学院
• 2:广东海洋大学广东省普通高校南海水产经济动物增养殖重点实验室
• 3:广东海洋大学广东省水产经济动物病原生物学及流行病学重点实验室

摘要(Abstract)：

采用RACE-PCR克隆卵形鲳鲹(Trachinotus ovatus)肉碱棕榈酰基转移酶Ⅰ(carnitine palmitoyltransferaseⅠ,CPTⅠ)c DNA序列全长,并对其编码氨基酸进行生物信息学分析。结果表明,卵形鲳鲹CPTⅠ基因(Gen Bank登录号KP987456)c DNA序列全长2 841 bp,其开放阅读框(ORF)为2 363 bp,编码787个氨基酸,3'非编码区(URT)335 bp,5'非编码区142 bp;生物信息预测显示CPTⅠ基因编码的蛋白无信号肽序列,脂溶指数高达85.63,亲水性平均值(GRAVY)为-0.213,具有2个跨膜区螺旋,在第312和367氨基酸残基处存在N-糖基化位点,在19个丝氨酸(Ser)、9个苏氨酸(Thr)和14个酪氨酸(Tyr)残基上可能发生磷酸化;二级结构中α螺旋(Alpha helix)占比例最大,为40.66%;该蛋白亚细胞定位预测其主要分布于细胞质和线粒体中;分子系统进化分析显示,卵形鲳鲹CPTⅠ蛋白与花鲈(Lateolabrax japonicas)的同源性最高,达94%,与大黄鱼(Larimichthys crocea)、金鲷(Sparus aurata)的次之,均为93%,与人(Homo sapiens)、鼠(Mus musculus)等的同源性较低(65%)。

关键词(KeyWords)： 卵形鲳鲹;肉碱棕榈酰基转移酶I;基因;克隆;生物信息分析

Abstract:

Keywords:

基金项目(Foundation): 国家海洋公益性行业科研专项(201205028);; 广东省海洋经济创新发展区域示范专项(GD2012-A01-007,GD2012-A02-003);; 广东省教育厅创新计划专项(2012KJCX0063);; 广西科技厅科技计划(桂科攻1222013-2)

作者(Author): 方玲玲,王忠良,陈刚,汤保贵,张健东,黄建盛,周晖

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