何军军,梁海鹰,陈崧,房晓宸,黄雪敏

• 1:广东海洋大学水产学院

摘要(Abstract)：

【目的】克隆马氏珠母贝(Pinctada martensii)肿瘤坏死因子受体相关死亡域蛋白(TRADD)基因,并分析其在各组织中的表达。【方法】利用cDNA末端快速扩增技术(RACE)克隆获得马氏珠母贝PmTRADD基因的c DNA全长序列,利用实时荧光定量PCR(qPCR)方法分析PmTRADD基因在马氏珠母贝不同组织中的表达模式。【结果与结论】PmTRADD包含5′非编码区101 bp,3′非编码区144 bp和开放阅读框(ORF)591 bp,编码196个氨基酸。序列分析表明,PmTRADD没有信号肽和跨膜结构域,C端含有一个死亡结构域(DEATH)。将PmTRADD死亡结构域的氨基酸序列与其他物种的TRADD死亡结构域序列进行比对,发现不同物种的TRADD死亡结构域序列同源性较低。PmTRADD在马氏珠母贝各组织中均有不同程度表达,在鳃组织中表达最高,肝胰腺次之,闭壳肌中基本无表达。

关键词(KeyWords)： 马氏珠母贝;肿瘤坏死因子受体相关死亡域蛋白;基因克隆;实时荧光定量

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31472306);; 广东省海港建设与渔业产业发展专项(A201608B15)

作者(Author): 何军军,梁海鹰,陈崧,房晓宸,黄雪敏

参考文献(References)：

• [1]陆晓娟,杨帅,罗少杰,等.马氏珠母贝前列腺素E合酶2基因PGES-2的克隆与表达分析[J].基因组学与应用生物学,2019,39(4):1-14.
• [2]吴羽媛,郭志颖,梁海鹰,等.马氏珠母贝PmLec-8基因的克隆与表达分析[J].广东海洋大学学报,2017,37(4):1-7.
• [3]KIM J Y,LEE J Y,KIM D G,et al.TRADD is critical for resistance to TRAIL-induced cell death through NF-κBactivation[J].FEBS Letters,2011,585(14):2144-2150.
• [4]HSU H,SHU H B,PAN M G,et al.TRADD-TRAF2 and TRADD-FADD interactions define two distinct TNFreceptor 1 signal transduction pathways[J].Cell,1996,84(2):299-308.
• [5]HSU H,XIONG J,GOEDDEL D V.The TNF receptor1-associated protein TRADD signals cell death and NF-κB activation[J].Cell,1995,81(4):495.
• [6]ASHKENAZI,A.Death receptors:signaling and modulation[J].Science,1998,281(5381):1305-0308.
• [7]POBEZINSKAYA Y L,KIM Y S,CHOKSI S,et al.The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors[J].Nature Immunology,2008,9(9):1047-1054.
• [8]CHEN,G.TNF-R1 signaling:a beautiful pathway[J].Science,2002,296(5573):1634-1635.
• [9]MICHEAU O,JüRG TSCHOPP.Induction of TNFreceptor I-mediated apoptosis via two sequential signaling complexes[J].Cell,2003,114(2):181-190.
• [10]WAJANT H,PFIZENMAIER K,SCHEURICH P.Tumor necrosis factor signaling[J].Cell Death&Differentiation,2003,10(1):45-65.
• [11]SWARUP V,GHOSH J,DAS S,et al.Tumor necrosis factor receptor-associated death domain mediated neuronal death contributes to the glial activation and subsequent neuroinflammation in Japanese encephalitis[J].Neurochemistry International,2008,52(7):1310-1321.
• [12]SWARUP V,DAS S,GHOSH S,et al.Tumor necrosis factor receptor-1-induced neuronal death by TRADDcontributes to the pathogenesis of Japanese encephalitis[J].Journal of Neurochemistry,2007,103(2):771-783.
• [13]CHIO I I C,SASAKI M,GHAZARIAN D,et al.TRADD contributes to tumour suppression by regulating ULF-dependent p19Arf ubiquitylation[J].Nature Cell Biology,2012,14(6):625-633.
• [14]XIE P.TRAF molecules in cell signaling and in human diseases[J].Journal of Molecular Signaling,2013,8(1):7.
• [15]WANG F,WENG H,QUON M J,et al.FADD dominant negative dissipates the proapoptotic signalosome of the unfolded protein response in diabetic embryopathy[J].American Journal of Physiology-Endocrinology And Metabolism,2015,309(10):861-873.
• [16]KRENZ M.Cell biology of ischemia/reperfusion injury[J].International Review of Cell&Molecular Biology,2012,298:229.
• [17]KAZUTAKA T,TAKAHASHI K G.Mechanisms and immunological roles of apoptosis in molluscs[J].Current Pharmaceutical Design,2008,14(2):131-137.
• [18]SOKOLOVA I M.Apoptosis in molluscan immune defense[J].Invertebrate Survival Journal,2009,6(1):49-58.
• [19]ROMERO A,NOVOA B,FIGUERAS A.The complexity of apoptotic cell death in mollusks:An update[J].Fish&Shellfish Immunology,2015,46(1):79-87.
• [20]WANG W,WU Y,LEI Q.Deep transcriptome profiling sheds light on key players in nucleus implantation induced immune response in the pearl oyster Pinctada martensii[J].Fish&Shellfish Immunology,2017,118(2):489-495.
• [21]MARIE-CéCILE MICHALLET,MEYLAN E,ERMOLAEVA M A,et al.TRADD protein is an essential component of the RIG-like helicase antiviral pathway[J].Immunity,2008,28(5):651-661.
• [22]XIANG Z,XIAO S,WANG F,et al.Cloning,characterization and comparative analysis of four death receptorTNFRs from the oyster Crassostrea hongkongensis[J].Fish&Shellfish Immunology,2016,59:288-297.
• [23]QU F,XIANG Z,ZHOU Y,et al.A molluscan TNFreceptor-associated factor 2(TRAF2)was involved in host defense against immune challenges[J].Fish&Shellfish Immunology,2017,71:105-115.
• [24]吴羽媛,梁海鹰,阮尧,等.马氏珠母贝TLR13基因的克隆与组织表达分析[J].基因组学与应用生物学,2018,37(1):238-246.
• [25]雷超,贝伟烈,郑哲,等.马氏珠母贝B型清道夫受体PmSR-B基因克隆与表达性分析[J].广东海洋大学学报,2017,37(1):7-14.