焦钰,阎芳,杜晓东,黄荣莲,王庆恒,邓岳文

• 1:广东海洋大学水产学院

摘要(Abstract)：

Su(H)基因(Suppressor of Hairless gene)对生物体细胞的分化、增殖和凋亡起重要的调控作用。根据马氏珠母贝(Pinctada martensii)转录组数据库中注释为Su(H)的unigene序列设计基因特异性引物,应用cDNA末端快速扩增(RACE)技术克隆获得了马氏珠母贝Su(H)[pm-Su(H)]基因cDNA全长序列。结果表明:pm-Su(H)基因全长3 830 bp,其中开放阅读框含有1 920 bp,编码640个氨基酸残基,5'UTR为1 568bp,3'UTR为342 bp,含有27 bp polyA;预测其分子质量为70.6 ku,等电点为7.38;多序列比对显示,pm-Su(H)与其他物种的Su(H)有较高的保守性,与牡蛎(Crassostrea gigas)的同源序列高达80%;荧光定量PCR分析表明,pm-Su(H)在马氏珠母贝闭壳肌、鳃、珍珠囊、外套膜、肝胰脏、性腺、足、血淋巴等8组织中均有表达,其中以性腺中表达量最高,其次是珍珠囊和外套膜。

关键词(KeyWords)： 马氏珠母贝;Su(H)基因;基因克隆;基因序列;基因表达;组织

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31272635,31201023);; 广东省自然科学基金(S2012040008042);; 广东省教育厅育苗工程(2012LYM_0074);; 广东海洋大学博士启动项目(1212318)

作者(Author): 焦钰,阎芳,杜晓东,黄荣莲,王庆恒,邓岳文

参考文献(References)：

• [1]Baron M.An overview of the Notch signalling pathway[J].Semin Cell Dev Biol,2003,14(2):113-119.
• [2]Gazave E,Lapebie P,Richards G S,et al.Origin and evolution of the Notch signalling pathway:an overview from eukaryotic genomes[J].BMC Evol Biol,2009,9(1):249.
• [3]Lecourtois M,Chweisguth F S.Role of suppressor of hairless in the delta-activated Notch signaling pathway[J].Perspect Dev Neurobiol,1997,4(4):305-311.
• [4]Lai E C.Keeping a good pathway down:transcriptional repression of Notch pathway target genes by CSL proteins[J].EMBO Rep,2002,3(9):840-845.
• [5]Maier D,Kurth P,Schulz A,et al.Structural and functional analysis of the repressor complex in the Notch signaling pathway of Drosophila melanogaster[J].Mol Biol Cell,2011,22(17):3242-3252.
• [6]Yuan Z,Friedmann D R,VanderWielen B D,et al.Characterization of CSL(CBF-1,Su(H),Lag-1)mutants reveals differences in signaling mediated by Notch1 and Notch2[J].J Biol Chem,2012,287(42):34904-34916.
• [7]Furriols M,Bray S.Dissecting the mechanisms of suppressor of hairless function[J].Dev Biol,2000,227(2):520-532.
• [8]Bray S,Furriols M.Notch pathway:making sense of suppressor of hairless[J].Curr Biol,2001,11(6):R217-221.
• [9]Zanotti S,Canalis E.Notch signaling in skeletal health and disease[J].Eur J Endocrinol,2013,168(6):R95-103.
• [10]Shimizu T,Tanaka T,Iso T,et al.Notch signaling pathway enhances bone morphogenetic protein 2(BMP2)responsiveness of Msx2 gene to induce osteogenic differentiation and mineralization of vascular smooth muscle cells[J].J Biol Chem,2011,286(21):19138-19148.
• [11]Zanotti S,Smerdel-Ramoya A,Stadmeyer L,et al.Notch inhibits osteoblast differentiation and causes osteopenia[J].Endocrinology,2008,149(8):3890-3899.
• [12]Jiao Y,Wang H,Du X D,et al.Dermatopontin,a shell matrix protein gene from pearl oyster Pinctada martensii,participates in nacre formation[J].Biochem Biophys Res Commun,2012,425(3):679-683.
• [13]Pursglove S E,Mackay J P.CSL:a notch above the rest[J].Int J Biochem Cell Biol,2005,37(12):2472-2477.
• [14]Johnson J E,Macdonald R J.Notch-independent functions of CSL[J].Curr Top Dev Biol,2011,97:55-74.
• [15]Zhao X X,Wang Q H,Jiao Y,et al.Identification of genes potentially related to biomineralization and immunity by transcriptome analysis of pearl sac in pearl oyster Pinctada martensii[J].Mar Biotechnol(NY),2012,14(6):730-739.
• [16]Larkin M K,Holder K,Yost C,et al.Expression of constitutively active Notch arrests follicle cells at a precursor stage during Drosophila oogenesis and disrupts the anterior-posterior axis of the oocyte[J].Development,1996,122(11):3639-3650.
• [17]Westbroek P,Marin F.A marriage of bone and nacre[J].Nature,1998,392(6679):861-862.