莫俊杰,胡汉桥,梁钾贤,陈静思,许良珠,吴仁锋

• 1:华南农业大学农学院
• 2:广东海洋大学农学院生物技术系
• 3:武汉蔬菜科学研究所

摘要(Abstract)：

为有效地保存抗病种质资源,对主栽的芋头品系进行疫病的抗病性鉴定及遗传多样性分析。从我国南方4个芋头产区收集60多个芋头品系,采用改进的疫霉菌抗病性鉴定方法,经过2 a的抗性鉴定,从中鉴定出6个抗病品系,9个中抗品系。选取全部的抗病品系及分别代表不同地区的感病品系37个,用SSR进行遗传多样性分析。聚类分析结果表明,芋头品系可分为5个类群。15个抗病品系分属不同类群,野生芋和食叶芋属于第5类群,与其它类群的亲缘关系较远。

关键词(KeyWords)： 芋头;疫病;遗传多样性;抗病性鉴定

Abstract:

Keywords:

基金项目(Foundation): 《水生蔬菜主要病虫害无害化综合防控技术研究》公益性行业(农业)科研专项经费子课题分项目(200903017-08)

作者(Author): 莫俊杰,胡汉桥,梁钾贤,陈静思,许良珠,吴仁锋

参考文献(References)：

• [1]Irwin S V,Kaufusi P,Banks K,et al.Molecular characterization oftaro(Colocasia esculenta)using RAPD markers[J].Euphytica,1998,99(3):183-189.
• [2]Lebot V,Aradhya M.Isozyme variation in taro[Colocasia esculenta(L.)Schott]from Asia and the Pacific[J].Euphytica,1991,56(1):55-66.
• [3]Kreike C M,Van Eck H J,Lebot V.Genetic diversity of taro,Colocasia esculenta(L.)Schott,in Southeast Asia and the Pacific[J].Theoretical and Applied Genetics,2004,109(4):761-768.
• [4]Singh D,Mace E S,Godwin I D,et al.Assessment and rationalizationof genetic diversity of Papua New Guinea taro(Colocasia esculenta)using SSR DNA fingerprinting[J].Genetic Resources and CropEvolution,2008,55(6):811-822.
• [5]龙雯虹,许明辉,张应华.云南芋头种质资源RAPD分子标记的初步研究[J].云南农业大学学报,2001,16(4):274-276.
• [6]Thankappan M.Leaf blight of taro-a review[J].Journal of Root Crops1985,11(1/2):223-236.
• [7]Misra R S,Chowdhury S R.Phytophthora leaf blight disease oftaro[C]//Tech Bull Ser 21.Trivandrum:Central Tuber Crops ResearchInstitute,1997:32.
• [8]Brooks F E.Detached-leaf bioassay for evaluating taro resistance toPhytophthora colocasiae[J].Plant Disease,2008,92(1):126-131.
• [9]Sharma K,Mishra A K,Misra R S.Identification and characterizationof differentially expressed genes in the resistance reaction in taroinfected with P.colocasiae[J].Molecular Biology Reports,2009,36(6):1291-1297.
• [10]Sharma K,Mishra A K,Misra R S.Analysis of AFLP Variation ofTaro Population and Markers Associated with Leaf Blight ResistanceGene[J].Academic Journal of Plant Sciences,2008,1(3):42-48.
• [11]李智军,龙卫平,郑锦荣,等.广东辣椒疫霉菌分离鉴定及其致病力和生理小种分化研究[J].华南农业大学学报,2007,28(1):50-54.
• [12]Michiels A,Van den Ende W,Tucker M,et al.Extraction ofhigh-quality genomic DNA from latex-containing plants[J].Analytical Biochemistry,2003,315(1):85-89.
• [13]Emma S.Mace,Ian D.Godwin.Development and characterization ofpolymorphic microsatellite markers in taro(Colocasia esculenta)[J].Genome,2002,(45):823-832.
• [14]Birhman R K,Singh B P.Path-coefficient analyses and geneticparameters of the components of field resistance of potatoes to lateblight[J].Annals of Applied Biology,1995,127(2):353-362.
• [15]Iwaro A D,Sreenivasan T N,Umagaran P.Foliar resistance toPhytophthora palmivora as an indicator of pod resistance inTheobroma cacao[J].Plant Disease,1997,81(6):619-624.
• [16]Zhang G M.Study on the chromosome number of the taro(Colocasiaesculenta Schott.)in China[J].Acta Horticulturae Sinica,1984,11(3):187-190.
• [17]Quero-Garcia J,Noyer J L,Perrier X,et al.A germplasmstratification of taro(Colocasia esculenta)based on agro-morphological descriptors,validation by AFLP markers[J].Euphytica,2004,137(3):387-395.
• [18]Caillon S,Quero-Garcia J,Lescure J P,et al.Nature of taro(Colocasia esculenta(L.)Schott)genetic diversity prevalent in aPacific Ocean island,Vanua Lava,Vanuatu[J].Genetic Resourcesand Crop Evolution,2006,53(6):1273-1289.
• [19]Hamza S,Hamida W B,Rebai A,et al.SSR-based genetic diversityassessment among Tunisian winter barley and relationship withmorphological traits[J].Euphytica,2004,135(1):107-118.