桂良超,麦浩彬,迟淑艳,周文豪,李永娟,谭北平,董晓慧,杨奇慧,刘泓宇,章双

• 1:广东海洋大学水产动物营养实验室
• 2:广东省海产无脊椎动物科技创新中心
• 3:北京英惠尔生物技术有限公司

摘要(Abstract)：

【目的】探讨高蛋白酵母培养物替代鱼粉对凡纳滨对虾(Litopenaeusvannamei)生长性能、血清生化指标、非特异性免疫力和抗病力的影响。【方法】在基础饲料中分别添加质量分数0、2.5%、5.0%和7.5%酵母培养物(记为Y0、Y2.5、Y5.0和Y7.5组),分别替代质量分数0、10%、20%和30%的鱼粉,配制4种等氮等脂饲料,饲喂凡纳滨对虾(初始体质量0.70±0.03 g)56 d后,测定对虾生长性能、血清生化及免疫酶指标;用哈维氏弧菌(Vibrioharveyi)攻毒,测定对虾累计死亡率。【结果】Y2.5和Y5.0组凡纳滨对虾增重率和特定生长率与Y0组的差异无统计学意义(P> 0.05),Y5.0组凡纳滨对虾摄食率显著高于Y0和Y2.5组(P <0.05);Y5.0组凡纳滨对虾血清甘油三酯含量显著低于Y0组(P<0.05),Y7.5组凡纳滨对虾血清谷丙转氨酶含量显著高于Y0、Y2.5组(P <0.05),血清总胆固醇显著低于Y0、Y2.5组(P <0.05);酵母培养物替代饲料中20%和30%的鱼粉可显著提高对虾攻毒前肝胰腺超氧化物歧化酶、过氧化物酶活性(P <0.05),Y7.5组对虾肝胰腺丙二醛含量在哈维氏弧菌(Vibrio harveyi)攻毒后显著低于Y0组(P <0.05);攻毒7 d后,Y7.5组凡纳滨对虾累积死亡率显著低于Y0组(P<0.05)。【结论】酵母培养物替代鱼粉比例小于20%时,不会显著影响凡纳滨对虾生长性能;替代比例为30%时,生长受抑制。当替代量比例为30%时可显著提高凡纳滨对虾非特异性免疫力和抗病力。

关键词(KeyWords)： 酵母培养物;凡纳滨对虾;生长性能;免疫力;抗病力

Abstract:

Keywords:

基金项目(Foundation): 广东省高等学校高层次人才项目[粤财教(2013)246];; 高校重大科研项目培育计划(GDOU2013050302)

作者(Author): 桂良超,麦浩彬,迟淑艳,周文豪,李永娟,谭北平,董晓慧,杨奇慧,刘泓宇,章双

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