诸德斐,杨润青,宋培钦,魏东

• 1:华南理工大学食品科学与工程学院

摘要(Abstract)：

【目的】制备富含岩藻黄素的三角褐指藻(Phaeodactylum tricornutum)生物质。【方法】采用470 L室内管道光生物反应器系统,研究自养培养下光源、调光策略、补料培养操作条件对于细胞生长、生物量浓度和岩藻黄素积累的影响。【结果】在采用LED灯、梯度提升照度及重复补料半连续培养模式下,三角褐指藻细胞密度、生物量和岩藻黄素产率最高分别可达3.16×10~7 mL~(-1)、1.64 g/L和1.90 mg/(L·d),分别是日光灯为光源条件下的2.77倍、3.09倍和2.38倍(P <0.01)。【结论】在管道光生物反应器中获得的自养培养条件,可强化三角褐指藻生物量和岩藻黄素积累,为三角褐指藻积累岩藻黄素的室内可控规模化技术开发提供指导。

关键词(KeyWords)： 三角褐指藻;岩藻黄素;管道光生物反应器;光源;LED灯;补料培养

Abstract:

Keywords:

基金项目(Foundation): 广东省公益研究与能力建设项目(2015A020216003)

作者(Author): 诸德斐,杨润青,宋培钦,魏东

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