刘慧玲,罗鹏,杨世平,李广聪,莫嘉文,王蔚

• 1:广东海洋大学水产学院
• 2:中国科学院海洋生物资源可持续利用重点实验室
• 3:中国科学院南海海洋研究所
• 4:国家海洋局第三海洋研究所

摘要(Abstract)：

用对虾饲料培养基从健康凡纳滨对虾肠道分离出500株黏附细菌,以产淀粉酶、脂肪酶和蛋白酶能力为指标,筛选出产该3种消化酶的细菌90株,占总菌株的18%。对其中生长较快的69株进行16S rDNA基因测序,确定其分类地位。结果显示,69株菌分别属于不动杆菌属(Acinetobacter)、芽孢杆菌属(Bacillus)、葡萄球菌属(Staphylococcus)、假交替单胞菌属(Pseudoalteromonas)、气单胞菌属(Aeromonas)、嗜盐单胞菌属(Halomonas)、利斯顿氏菌属(Listonella)、莫拉氏菌属(Moraxella)等,其中数量最多是芽胞杆菌属,占鉴定细菌总数的53.62%,数量最少是气单胞菌属和嗜盐单胞菌属,均占鉴定细菌总数的2.90%。表明对虾肠道黏附菌群中具有较多能分泌多种消化酶的细菌,可进一步开发为促进对虾消化功能的益生菌。

关键词(KeyWords)： 凡纳滨对虾;肠道细菌;胞外酶

Abstract:

Keywords:

基金项目(Foundation): 中科院南海海洋研究所青年前沿重点项目(SQ200801);; 广东省海洋渔业科技推广专项项目(A2010005-048(c));; 国家自然科学基金(30800857)

作者(Author): 刘慧玲,罗鹏,杨世平,李广聪,莫嘉文,王蔚

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