张兴志,何苹萍,官俊良,韦嫔媛,江林源,张立,陈泳先,罗帮,马元,李蔚,李满园,李恒慧,彭金霞

• 1:广西水产科学研究院广西水产遗传育种与健康养殖重点实验室
• 2:合浦县水产技术推广站

摘要(Abstract)：

【目的】研究香港牡蛎（Crassostrea hongkongensis）对低氧的耐受性及复氧后的恢复能力，为香港牡蛎养殖及耐低氧品系选育提供参考。【方法】开展溶解氧分别为0.5、1.5、2.5 mg/L低氧胁迫72 h及复氧24 h的实验，分析其鳃、闭壳肌的抗氧化能力及能量代谢相关酶活性的变化情况。【结果】1）低氧胁迫6 h时，0.5 mg/L及1.5 mg/L两组香港牡蛎总抗氧化能力（T-AOC）、糖原含量、丙酮酸激酶（PK）活性和磷酸果糖激酶（PFK）活性显著降低（P <0.05），磷酸烯醇式丙酮酸激酶（PEPCK）活性与对照组差异不显著（P> 0.05）;2）低氧胁迫72 h时，T-AOC、糖原含量以及能量代谢酶PK、PFK、PEPCK活性显著升高（P <0.05）;3）复氧24 h后，1.5 mg/L和2.5 mg/L两个低氧组牡蛎的T-AOC,PK酶及PFK酶活性基本恢复至初始水平，与对照组相比差异不显著（P> 0.05）,0.5 mg/L低氧组牡蛎各项指标仍显著低于对照组，3个低氧组PEPCK酶活性均显著高于对照组（P <0.05）。【结论】低氧显著影响香港牡蛎的抗氧化能力和能量代谢相关的酶活性，72 h内，牡蛎对1.5、2.5 mg/L低氧胁迫补偿适应能力较佳，但对0.5 mg/L极端低氧胁迫的补偿代谢能力较差。

关键词(KeyWords)： 香港牡蛎;低氧胁迫;复氧;抗氧化能力;能量代谢

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划“海洋农业与淡水渔业科技创新”重点专项（2022YFD2401203,2022YFD2401205）;; 广西重点研发项目（桂科AB21196030）;; 广西自然科学基金（2023GXNSFBA026311）;; 广西水产遗传育种与健康养殖重点实验室自主研究课题（23-A-01-03,22-A-01-01）;; 国家贝类产业技术体系综合试验站项目（CARS-49）;; 国家现代农业产业技术体系广西创新团队项目（nycytxgxcxtd-2023-14-05）;; 广西农业科技自筹经费项目（Z202290）

作者(Author): 张兴志,何苹萍,官俊良,韦嫔媛,江林源,张立,陈泳先,罗帮,马元,李蔚,李满园,李恒慧,彭金霞

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