刘昱璇,冯子齐,王震,余方苗

• 1:浙江海洋大学食品与药学学院/浙江省海洋生物医用制品工程技术研究中心

摘要(Abstract)：

【目的】开发厚壳贻贝（Mytilus coruscus）乙醇脱氢酶（Alcohol dehydrogenase,ADH）激活肽，并探究其与ADH作用的分子机制。【方法】以体外ADH激活率为指标，通过凝胶柱层析色谱、反向液相色谱法（RP-LC）分离纯化分子质量小于1 ku的厚壳贻贝活性肽（Mytilus coruscus peptides,MCP），利用液相色谱串联质谱技术（LC-MS/MS）对高ADH激活性的肽组分进行鉴定，并通过分子对接技术探究其与ADH间的相互作用。【结果】MCP经色谱分离得到活性组分Y-I-I，鉴定并筛选出其中3条与ADH对接结合能较低的活性肽序列PPLYE、PPLYQ和APPLYQ，其中活性肽PPLYE体外ADH激活作用最佳，在PPLYE质量浓度为2.0 mg/mL时，ADH激活率达到77.35%，且呈剂量依赖性。分子对接结果显示，PPLYE可结合至ADH活性中心附近的疏水空腔内，通过疏水作用和氢键与受体蛋白ADH形成稳定复合物，其结合能最低，为-35.58 kJ/mol。【结论】PPLYE作为最佳的ADH激活肽，可通过疏水作用和氢键与ADH相互作用形成稳定的复合物来提高ADH活性，本研究为厚壳贻贝资源的开发利用及ADH激活肽的开发提供依据。

关键词(KeyWords)： 乙醇脱氢酶;厚壳贻贝活性肽;分离纯化;分子对接

Abstract:

Keywords:

基金项目(Foundation): 浙江省属高校基本科研业务费（2021JZ011）

作者(Author): 刘昱璇,冯子齐,王震,余方苗

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