王成,赖卓欣,宋欣霖,钟如卓,郑哲,王庆恒

• 1:广东海洋大学水产学院
• 2:广东省珍珠养殖与加工工程技术研究中心
• 3:广东省珍珠科技创新中心
• 4:广东省水产动物病害防控与健康养殖重点实验室

摘要(Abstract)：

【目的】克隆马氏珠母贝（Pinctada fucata martensii）新的凝集素分子基因，命名为Perlucin，研究在低温胁迫下马氏珠母贝Perlucin的表达以及与抗低温性状相关的单核苷酸多态性（SNP）位点。【方法】根据马氏珠母贝基因组中Perlucin基因序列设计引物，克隆Perlucin基因全长；用生物信息学方法分析Perlucin的结构和理化特征；设计17和22℃（对照）2个温度组，对马氏珠母贝进行低温胁迫实验，用实时荧光定量PCR检测低温胁迫下Perlucin表达量的变化；筛选和比较分析马氏珠母贝耐低温选育系（R）F3和北部湾野生群体（W）的Perlucin外显子区的SNP位点和单倍型。【结果】马氏珠母贝Perlucin全长631 bp，编码152个氨基酸；包含1个信号肽和1个C型凝集素结构域。同源分析表明，马氏珠母贝Perlucin与紫贻贝（Mytilus galloprovincialis）Perlucin的亲缘性最近。Perlucin在鳃中表达量最高，其次为足和肝胰腺。低温胁迫时，鳃组织中Perlucin基因在17℃低温组的表达量呈先升高后降低的趋势，在12 h时达到最高并显著高于22℃对照组（P <0.05），表明Perlucin可能参与马氏珠母贝的低温响应过程。对Perlucin外显子区的SNP进行分析，共得到30个SNP，其中13个SNP位点的基因型频率在R和W群体间差异显著（P <0.05）。【结论】Perlucin是参与调节马氏珠母贝低温适应过程中的候选基因，筛选出两个SNP位点g.40078856、g.40078945。

关键词(KeyWords)： 马氏珠母贝;Perlucin;基因克隆;低温胁迫;SNP

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金（2023A1515030048）;; 广东省普通高校重点领域专项（2020ZDZX1045）;; 广东省现代农业产业技术体系-贝藻类产业创新团队项目（KJ146）;; 湛江市科技计划（2022A01010）

作者(Author): 王成,赖卓欣,宋欣霖,钟如卓,郑哲,王庆恒

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