刘连为;陈新军;许强华;王从军;余为;

• 1:上海海洋大学海洋科学学院
• 2:上海海洋大学大洋生物资源开发和利用上海市高校重点实验室
• 3:上海海洋大学大洋渔业资源可持续开发省部共建教育部重点实验室

摘要(Abstract)：

为准确检测柔鱼(Ommastrephes bartramii)、茎柔鱼(Dosidicus gigas)与阿根廷滑柔鱼(Illex argentinus)的种间遗传差异,对线粒体16S rRNA、细胞色素b(Cytb)与编码核糖体大亚基的基因(28S rDNA)片段序列进行测定。经比对获得同源片段序列的长度分别为444、430、464 bp,其中16S rRNA与28S rDNA基因片段上分别存在3处和47处碱基插入/缺失。核苷酸组成分析表明:3种柔鱼在3个基因片段上的核苷酸组成差异不显著,在线粒体2个基因片段上的A+T含量(16S rRNA:69.90%、72.01%、74.66%;Cytb:63.61%、68.91%、71.65%)均明显高于G+C含量(16S rRNA:30.10%、27.99%、25.34%;Cytb:36.39%、31.09%、28.35%),而在28S rDNA基因片段上的A+T含量(37.16%、36.74%、38.29%)明显低于G+C含量(62.84%、63.26%、61.71%)。3种柔鱼在28S rDNA基因片段上检测到的核苷酸替代率最低,为6.68%,而蛋白质编码基因Cytb核苷酸替代率最高,为20.93%,核苷酸替代均发生在密码子第3位点上,而且未引起氨基酸替代。基于邻接法、最大简约法与最大似然法重建的系统树显示,柔鱼与茎柔鱼的亲缘关系较近。根据Cytb基因片段序列分析,柔鱼与茎柔鱼和阿根廷滑柔鱼的分歧时间分别为653⁷90万a和765790万a和765⁹25万a,种间分化事件发生在中新世至上新世间。

关键词(KeyWords)： 柔鱼;线粒体基因;核核糖体基因;序列分析;分歧时间

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(41276156);; 上海市科委重大项目(12231203900);; 国家发改委产业化专项(2159999)

作者(Authors): 刘连为;陈新军;许强华;王从军;余为;

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