刘岭萍,谭玉冰,薛明,王优阳,陈丽莹,梁喜,华郡,梁华芳,温崇庆

• 1:广东海洋大学水产学院

摘要(Abstract)：

【目的】从对虾肠道分离鉴定拮抗病原弧菌的益生菌，对其生物安全性及对凡纳滨对虾（Litopenaeus vannamei）幼体弧菌病的控制效果进行评价，以促进益生菌对对虾弧菌病的防治效果。【方法】利用平板划线法从虾肠分离细菌，根据拮抗病原弧菌活性筛选潜在益生菌株，结合表型特征、16S rRNA和rpoB基因序列对目标菌株进行鉴定，利用纸片扩散法测试其对17种抗菌药物敏感性。通过水体添加目标菌评价其对凡纳滨对虾幼体感染副溶血弧菌（Vibrio parahaemolyticus）的生物防治效果。【结果】从对虾肠道分离筛选得到一株对副溶血弧菌、哈维氏弧菌（Vibrio harveyi）和坎氏弧菌（Vibrio campbellii）均有明显拮抗作用的菌株LJ;LJ不溶血且无多重耐药性，被鉴定为枯草芽孢杆菌（Bacillus subtilis）。水体添加1×10~5和1×10~6 cfu/mL LJ均可显著提高感染副溶血弧菌的对虾幼体存活率和变态率（P <0.05），且高剂量处理组效果更明显；未添加病原菌条件下，高浓度（5×10~7 cfu/mL） LJ对幼体无害，并能显著促进幼体存活和变态。【结论】对虾肠源枯草芽孢杆菌LJ生物安全性高，可有效拮抗病原弧菌和提高感染副溶血弧菌凡纳滨对虾幼体的存活率。

关键词(KeyWords)： 枯草芽孢杆菌LJ;益生菌;弧菌;拮抗;生物防控;凡纳滨对虾;药敏

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划“蓝色粮仓”科技创新重点专项(2020YFD0900205);; 广东省南美白对虾现代种业产业园项目(K22218);; 廉江市养虾集团有限公司委托项目(003)

作者(Author): 刘岭萍,谭玉冰,薛明,王优阳,陈丽莹,梁喜,华郡,梁华芳,温崇庆

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