基于线粒体16SrRNA基因的14种造礁石珊瑚系统发育关系分析Phylogenetic Analysis for 14 Species of Reef-building Corals Based on 16SrRNA genes
廖宝林,肖宝华,杨小东,谢子强
摘要(Abstract):
通过16SrRNA基因特异扩增测序,对惠州大亚湾大辣甲岛和小辣甲岛附近海域14种造礁石珊瑚16SrRNA基因片段序列进行了比较分析。结果表明,该片段序列的平均A+T含量为63.5%,所有物种的序列碱基AT含量大于GC含量,序列都处于高度饱和的状态。14种造礁石珊瑚的平均遗传距离为0.179。采用NJ、ML和ME法构建系统发育树,结果显示蜂巢珊瑚科单独作为分支处于发育树的基部,而第二支分支包括菌珊瑚科、鹿角珊瑚科、滨珊瑚科和枇杷珊瑚科。裸肋珊瑚科的腐蚀刺柄珊瑚聚到了蜂巢珊瑚科中,与传统形态学分类存在差异,提示造礁石珊瑚表型的变异性可能对传统分类存在影响。
关键词(KeyWords): 造礁石珊瑚;16SrRNA;分子系统学
基金项目(Foundation): 广东省公益研究与能力建设专项(K15216);; 广东省海洋渔业科技推广专项(A201308E02)
作者(Author): 廖宝林,肖宝华,杨小东,谢子强
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