地衣芽孢杆菌在异育银鲫日粮中的应用Application of Bacillus licheniformis to the Feed of Allogynogenetic crucian carp
刘波,刘文斌,王恬
摘要(Abstract)：
在异育银鲫基础日粮中分别添加质量分数0.01%、0.02%、0.03%、0.04%的地衣芽孢杆菌,测定了鱼体的生长及食糜植酸酶活性等。结果表明:与对照组相比,添加量0.01%、0.02%、0.03%组的增重率分别显著提高了12.46%、18.59%、18.84%;添加量0.02%、0.03%组的磷表观消化率分别提高37.63%、35.03%(P<0.05);饵料系数降低了0.52、0.57(P<0.05),其他组差异不显著。与对照组相比,0.03%组食糜植酸酶活性提高50.79%、0.04%组肝体比降低20.91%(P<0.05),其他组差异不显著。因此在异育银鲫日粮中添加地衣芽孢杆菌提高了机体酶活性,促进了鱼体生长。
关键词(KeyWords)： 地衣芽孢杆菌;生产性能;植酸酶活性;肝体比;异育银鲫
基金项目(Foundation): 国际合作项目(U200214)
作者(Author): 刘波,刘文斌,王恬
参考文献(References)：
- [1]Kozasa M.Toyocerin(Bacillus toyoi)as growth promotor for animalfeeding[J].Microbiol Aliment Nutr,1986,164(1/4):351~358
- [2]Rengpipat S,Phianphank W,Piyatiratitivorakul S,et al.Effects of aprobiotic bacterium on black tiger shrimpPenaeus monodonsurvival andgrowth[J].Aquaculture,1998,167(3/4):301~313
- [3]刘克琳,何明清.益生菌对鲤鱼免疫功能影响的研究[J].饲料工业,2000,21(6):24~25
- [4]刘小刚,周洪琪,华雪铭,等.微生态制剂对异育银鲫消化酶活性的影响[J].水产学报,2002,26(5):448~451
- [5]陆文清,刘伟.固体发酵饲用植酸酶酶活的快速测定方法[J].饲料研究,2000(1):11~12
- [6]尾崎久雄.鱼类消化生理[M].上海:上海科技出版社,1983
- [7]Henderickx H K.Influence of the gut flora and of some growth-promotingfeed additives on nitrogen metabolism in pigs[J].Liteafock Prod Sci,1986(14):161~176
- [8]胡学智.芽孢杆菌的酶[J].工业微生物,1993,23(4):25~40;23(5):34~37
- [9]Gatesoupe F J.Bacillussp.spore as food additive for the rotifer Bra-chionus Plicalis:improvement of their bacterial environment and their di-etary value larval turbotlScophalmus maximusL.Fish nutrition in prac-tice[C].//4th international symposium of fish nutrition and feeding.Biarritz,France,June 24~27,1991
- [10]刘云高,胡未一.鱼用微生物饲料添加剂(8801)对鲤鱼促生长试验[J].四川农业大学学报1994,12(增刊):650~653
- [11]姚键.DM423菌粉制剂对虾殖中的应用[J].中国饲料,1994(3):25~26
- [12]Schafer A,Koppe W M.Effects of a microbial phytase on the utiliza-tion of native phos-phorus by carp in a diet based on soybean meal[J].Water science and technology,1995,31(10):149~155
- [13]Jackson L S,Li M H,Robison E H.Use of microbial phytaze in chan-nel catfishIctalurus puncatus diets to improve utilization of phosphorus[J].Journal of the world aquaculture society,1996,27(3):309~313
- [14]Paratryphon E,Howell R A,Soares J H Jr.Growth and mineral ab-sorption by striped bassMorone saxatilisfed a plant feedstuff based dietsupplemented with phytase[J].Journal of the world aquaculture soci-ety,1999,(302):161~173
- [15]Lei X G,kupk,Miller E R,Ullrey D I,et al.Supplemental microbialphytase improves bioavailability of dietary zinc to weanling pigs[J].Nutrition,1993,123(6):1117~1123
- [16]Knuckles B I,Kelzmicky D D,Berschat A A.Effect of phytase andpartially hydrolyzed phytase in Vitro protein digestibility[J].Food sci-ence,1985,50:1080~1082
- [17]曾虹.植酸酶的研究及在鱼饲料中应用前景[J].上海水产大学学报,1998,12(增刊):39~43
- [18]王亚茹,姚斌.枯草芽孢杆菌中性植酸酶的纯化和酶学性质[J].微生物学报,2001,41(2):198~203
- [19]柏建玲,莫树平,郑婉玲,等.地衣芽孢杆菌与其他微生物产酶能力的比较[J].饲料研究,2003(7):4~6
- [20]杨汉博.地衣芽胞杆菌BL15和BL19对黄鸡的营养、微生态效应研究[D]:[博士论文].雅安:四川农业大学,2002
- [21]华雪铭,周洪琪,张宇峰,等.饲料中添加壳聚糖和益生菌对暗纹东方鱼屯幼鱼生长及部分消化酶活性的影响[J].水生生物学报,2005,29(3):300~304
- [22]NanjiA A,Khettry U,Sadrzadeh S M.Lactobacillus feeding reducesendotoxemia and severity of experimental alcoholic liver(disease)[J].Proc Soc Exp Biol Med,1994,205(3):243~247