耐盐水稻新种质农艺性状鉴定及其耐盐性Agronomic Traits Identification of New Germplasm of Salt-tolerant Rice and Its Salt-tolerance
张皓炀,褚晶,王宇,涂雅琴,江行玉,谢青
摘要(Abstract):
【目的】提高耐盐水稻抽穗抗倒伏特性和产量。【方法】以矮秆耐盐水稻新种质“海稻21”(SR21)为研究对象,系统比较其与亲本“海稻86”(SR86)的农艺性状差异,同时用0、50、100、150 mmol/L NaCl处理幼苗,比较SR21、SR86、盐敏感IR29的盐响应。【结果】SR21早稻正常成熟而SR86表现不抽穗,SR21晚稻生育期比SR86短23 d,株高降低33.93%,分蘖数减少24.24%,理论产量提升16.39%,主要归因于穗粒数增加49.48%,其中两者的株高、分蘖数、理论产量和穗粒数差异显著(P<0.001)。盐胁迫对3个种质的生长均产生一定的抑制,但对SR21和SR86的生长抑制作用较小。与IR29相比,SR21和SR86均可保持较高的抗氧化酶活性,减轻盐胁迫对细胞造成的氧化损伤。此外,SR21和SR86具备较好的渗透调节物质水平,使其在盐胁迫下保持较强的持绿能力。总体而言,盐胁迫下SR21和SR86的活性氧清除能力类似。【结论】SR21株型在缩短生育期的同时实现了产量提升,其地上部激活抗氧化酶系统维持光合稳态,根系增强超氧化物歧化酶(SOD)及过氧化物酶(POD)活性提升耐盐性。
关键词(KeyWords): 水稻;矮杆;种质资源;农艺性状;盐胁迫
基金项目(Foundation): 广东省自然科学基金项目“矮杆非光敏海水稻耐盐和非光敏机理及其滨海滩涂栽培技术研究”(2023A1515012295);; 广东海洋大学科研启动项目(060302052320)
作者(Author): 张皓炀,褚晶,王宇,涂雅琴,江行玉,谢青
参考文献(References):
- [1]陈林,阿米娜·胡吉,孙阳阳.土壤盐渍化现状和快速检测方法研究[J].新疆有色金属, 2024, 47(3):90-91.
- [2]杨真,王宝山.中国盐渍土资源现状及改良利用对策[J].山东农业科学, 2015, 47(4):125-130.
- [3] YI S Q, ZUO W G, XU L, et al. Accumulation and migration of microplastics and its influencing factors in coastal saline-alkali soils amended with sewage sludge[J]. Ecotoxicology and Environmental Safety, 2023, 266:115597.
- [4] DAHLAWI S, NAEEM A, RENGEL Z, et al. Biochar application for the remediation of salt-affected soils:challenges and opportunities[J]. Science of the Total Environment, 2018, 625:320-335.
- [5] FU C C, KHAN M N, YAN J S, et al. Mechanisms of nanomaterials for improving plant salt tolerance[J]. Crop and Environment, 2023, 2(2):92-99.
- [6] TUTEJA N, PETER SINGH L, GILL S S, et al. Salinity stress:a major constraint in crop production[J]. Improving crop resistance to abiotic stress, 2012:71-96.
- [7] XIE X F, JI R T, XU Z Q, et al. Effect of salt-tolerant rice(Oryza sativa L.)cultivation on soil bacterial community and ecological function groups in coastal saline land[J].Applied Soil Ecology, 2024, 201:105511.
- [8] ALI M N, YEASMIN L, GANTAIT S, et al. Screening of rice landraces for salinity tolerance at seedling stage through morphological and molecular markers[J]. Physiology and Molecular Biology of Plants, 2014, 20(4):411-423.
- [9] KAKAR N, JUMAA S H, REDO?A E D, et al. Evaluating rice for salinity using pot-culture provides a systematic tolerance assessment at the seedling stage[J]. Rice,2019, 12(1):57.
- [10]徐晨,凌风楼,徐克章,等.盐胁迫对不同水稻品种光合特性和生理生化特性的影响[J].中国水稻科学, 2013, 27(3):280-286.
- [11]周振玲,林兵,周群,等.耐盐性不同水稻品种对盐胁迫的响应及其生理机制[J].中国水稻科学, 2023, 37(2):153-165.
- [12] RAZZAQUE S, HAQUE T, ELIAS S M, et al. Reproductive stage physiological and transcriptional responses to salinity stress in reciprocal populations derived from tolerant(Horkuch)and susceptible(IR29)rice[J]. Scientific Reports, 2017, 7:46138.
- [13] XIE Z Y, WANG C C, ZHU S B, et al. Characterizing the metabolites related to rice salt tolerance with introgression lines exhibiting contrasting performances in response to saline conditions[J]. Plant Growth Regulation, 2020, 92(2):157-167.
- [14] CHEN R S, CHENG Y F, HAN S Y, et al. Whole genome sequencing and comparative transcriptome analysis of a novel seawater adapted, salt-resistant rice cultivar-sea rice86[J]. BMC Genomics, 2017, 18(1):655.
- [15]李红宇.水稻田间试验实用手册[M]. 2版.北京:中国农业出版社, 2021.
- [16] GIANNOPOLITIS C N, RIES S K. Superoxide dismutases:I. occurrence in higher plants[J]. Plant Physiology, 1977, 59(2):309-314.
- [17] KLAPHECK S, ZIMMER I, COSSE H. Scavenging of hydrogen peroxide in the endosperm of Ricinus communis by ascorbate peroxidase[J]. Plant and Cell Physiology,1990, 31(7):1005-1013.
- [18] AEBI H. Catalase in vitro[M]//Methods in Enzymology.Academic Press, 1984, 105:121-126.
- [19]赵世杰,许长成,邹琦,等.植物组织中丙二醛测定方法的改进[J].植物生理学通讯, 1994(3):207-210.
- [20] BRADFORD M M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Analytical Biochemistry, 1976, 72:248-254.
- [21]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社, 2000.
- [22]祝一文,赵方贵,成云峰,等.'海稻86'耐盐碱胁迫生理机制的初步研究[J].青岛农业大学学报(自然科学版),2018, 35(1):32-39.
- [23]李婷,朱长波,李俊伟,等.海水胁迫对海稻86种子萌发和幼苗生长的影响[J].南方农业学报, 2018, 49(7):1297-1303.
- [24] XUE W Y, XING Y Z, WENG X Y, et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice[J]. Nature Genetics, 2008, 40(6):761-767.
- [25]罗会超.水稻抽穗期相关基因的研究进展[J].农业科学,2024, 14(4):388-392.
- [26] CHEN H D, XIE W B, HE H, et al. A high-density SNP genotyping array for rice biology and molecular breeding[J]. Molecular Plant, 2014, 7(3):541-553.
- [27] TSUJI H, TAOKA K I, SHIMAMOTO K. Regulation of flowering in rice:two florigen genes, a complex gene network, and natural variation[J]. Current Opinion in Plant Biology, 2011, 14(1):45-52.
- [28]郑迎霞,陈杜,魏鹏程,等.种植密度对贵州春玉米茎秆抗倒伏性能及籽粒产量的影响[J].作物学报, 2021(4):738-751.
- [29] LI Q, FU C F, LIANG C L, et al. Crop lodging and the roles of lignin, cellulose, and hemicellulose in lodging resistance[J]. Agronomy, 2022, 12(8):1795.
- [30] SASAKI A, ASHIKARI M, UEGUCHI-TANAKA M, et al. Green revolution:a mutant gibberellin-synthesis gene in rice[J]. Nature, 2002, 416(6882):701-702.
- [31]张庆,殷春渊,张洪程,等.水稻氮高产高效与低产低效两类品种株型特征差异研究[J].作物学报, 2010, 36(6):1011-1021.
- [32] SAHA S R, HASSAN L, HAQUE M A, et al. Genetic variability, heritability, correlation and path analyses of yield components in traditional rice(Oryza sativa L.)landraces[J]. Journal of the Bangladesh Agricultural University, 2019, 17(1):26-32.
- [33]胡雅杰,曹伟伟,钱海军,等.钵苗机插密度对不同穗型水稻品种产量、株型和抗倒伏能力的影响[J].作物学报, 2015, 41(5):743-757.
- [34]袁隆平.杂交水稻超高产育种[J].杂交水稻, 2000, 15(S2):14-16.
- [35] ASADA K. Production and scavenging of reactive oxygen species in chloroplasts and their functions[J]. Plant Physiology, 2006, 141(2):391-396.
- [36] HOSSAIN M A, HOQUE M A, BURRITT D J, et al. Proline protects plants against abiotic oxidative stress[M]//Oxidative Damage to Plants. Amsterdam:Elsevier, 2014:477-522.
- [37] CHEN H, ZHANG J Y, NEFF M, et al. Integration of light and abscisic acid signaling during seed germination and early seedling development[J]. Proceedings of the National Academy of Sciences of the United States of America, 2008, 105(11):4495-4500.
- [38] MURATA N, TAKAHASHI S, NISHIYAMA Y, et al. Photoinhibition of photosystem II under environmental stress[J]. Biochimica et Biophysica Acta(BBA):Bioenergetics,2007, 1767(6):414-421.