黎钊坪,李雁群,李燕文,岳瑶,郭桂筱,胡雪琼

• 1:广东海洋大学食品科技学院
• 2:广东海洋大学食品科技学院海洋药物研究所
• 3:广东省水产品加工与安全重点实验室

摘要(Abstract)：

【目的】对蛋白核小球藻进行富硒混和培养研究,为富硒小球藻保健品食品的开发提供基础。【方法】采用碳氮比营养优化及pH培养条件优化的方式提高生物量及胞内蛋白含量,并在此基础上进行硒富集培养,以荧光分光光度法检测细胞硒含量。【结果】当碳氮比(质量比)20∶1时,生物量达到16.8 g/L,细胞蛋白质质量分数为31.46%;当碳氮比15∶1时,生物量为16.1 g/L,蛋白质含量为43.69%;当碳氮比10∶1时,生物量为14.7g/L,蛋白含量为45.21%。按单位培养液蛋白质产量计,以碳氮比15∶1最佳。培养基起始pH值为5.5时,藻细胞生物量可以达到18.9 g/L,起始pH=5.5～6.5范围内,适合小球藻生长。在富硒试验中,当培养基亚硒酸钠质量浓度达10 mg/L及以下,对小球藻细胞生长无明显影响,高于20 mg/L抑制生长,高于80 mg/L会严重抑制生长;亚硒酸钠质量浓度为10 mg/L时,藻细胞内的硒含量达到54.9μg/g,总富集硒量为927.9μg/L培养液,硒的吸收效率为20.3%。【结论】蛋白核小球藻能在混养培养下得到较高的生物量且具有较高的富硒能力。

关键词(KeyWords)： 微藻;蛋白核小球藻;富硒;混养

Abstract:

Keywords:

基金项目(Foundation): 广东省国际合作项目（2017A050501038）

作者(Author): 黎钊坪,李雁群,李燕文,岳瑶,郭桂筱,胡雪琼

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