黄雪敏,梁华芳,薛明,余文智,李成聪,陈耀,温崇庆

• 1:广东海洋大学水产学院

摘要(Abstract)：

【目的】研究广东湛江地区凡纳滨对虾(Litopenaeus vannamei)烂尾病主要病原及其药敏特征。【方法】从对虾病灶部位分离纯化细菌,用2株代表菌株对健康虾进行创伤浸浴感染试验,分析菌株形态特征、生理生化特征以及16S rRNA和HSP60基因序列,以哈维氏弧菌(Vibrio harveyi)和坎氏弧菌(V. campbelli)溶血素基因特异引物扩增菌株基因组DNA,通过纸片扩散法测试菌株对17种药物的敏感性。【结果】从患病亲虾和养殖对虾分别分离获得编号2018B22和2018MZ1的代表菌,两株菌16Sr RNA和HSP60基因序列均与哈维氏弧菌最相似,且均可被哈维氏弧菌溶血素基因引物特异扩增,表型特征亦与哈维氏弧菌相近;两株菌均可引起凡纳滨对虾烂尾病,其中菌株2018B22的致病力较2018MZ1更强,而后者的耐药谱更广。【结论】湛江地区凡纳滨对虾烂尾病主要病原为哈维氏弧菌。

关键词(KeyWords)： 凡纳滨对虾;烂尾病;哈维氏弧菌;鉴定;药敏试验

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31502189);; 广东海洋大学2019年度创新强校专项资金(230419095)

作者(Author): 黄雪敏,梁华芳,薛明,余文智,李成聪,陈耀,温崇庆

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