陈希平,王亚楠,杨嘉惠,刘梦飞,张佳程,邓红进,田继业,谭北平,迟淑艳

• 1:广东海洋大学水产学院水产动物营养与饲料实验室
• 2:辽宁农业职业技术学院
• 3:保定冀中药业有限公司

摘要(Abstract)：

【目的】研究饲料中添加白术（Atractylodes macrocephala Koidz）提取物（EA）对低盐度（盐度0.6）养殖凡纳滨对虾（Litopenaeus vannamei）生长性能，肠道抗氧化、抗炎及抗细胞凋亡的影响。【方法】在基础饲料中分别加入白术提取物，其添加量分别为0、100、300、500、700、1 500及3 000 mg/kg，制备7种等氮等脂饲料。这些饲料配方分别被标记为EA0、EA100、EA300、EA500、EA700、EA1500、EA3000组。分别投喂初始体质量（0.23±0.00）g的幼虾56 d，分析对虾生长性能，肠道抗氧化能力，肠道炎症因子及围食膜因子基因的表达量，以及肠道细胞凋亡指数。【结果】1)EA700组、EA1500组和EA3000组对虾的终末体质量、增重率和特定生长率显著高于EA0组（P <0.05）;EA700组的蛋白质沉积率显著高于EA0组和EA100组（P <0.05）;EA700组对虾的肥满度和肠体比均显著高于EA0组（P <0.05）;2)EA700组对虾的对虾肠道超氧化物歧化酶、过氧化氢酶活性以及总抗氧化能力显著高于EA0组（P <0.05），丙二醛含量显著低于其余组（P <0.05）;3)EA700组对虾肠道il-1β、il-16、tnf-α和nfil-3表达量显著低于EA0组（P <0.05）;4)EA700组和EA1500组的对虾肠道细胞凋亡指数显著低其余组（P <0.05）。在本实验条件下，以增重率为评价指标，折线模型拟合分析显示，凡纳滨对虾饲料中白术提取物的最适添加量为699.13 mg/kg。【结论】饲料中添加适宜水平的白术提取物可促进低盐度养殖凡纳滨对虾的生长，提高其肠道抗氧化、抗炎及抗细胞凋亡能力。

关键词(KeyWords)： 凡纳滨对虾;低盐度;白术提取物;生长性能;抗氧化能力;细胞凋亡

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划(2023YFD2402000)

作者(Author): 陈希平,王亚楠,杨嘉惠,刘梦飞,张佳程,邓红进,田继业,谭北平,迟淑艳

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