周维,汤菊芬,高增鸿,甘桢,简纪常,吴灶和,丁燏

• 1:广东海洋大学水产学院
• 2:广东省水产经济动物病原生物学及流行病学重点实验室
• 3:仲恺农业工程学院

摘要(Abstract)：

根据Gen Bank中公布的哈维氏弧菌qnr基因序列设计引物扩增哈维氏弧菌qnr序列,将其插入p ET-32a质粒,构建原核表达载体p ET32-qnr,并对诱导温度、时间、IPTG浓度等条件进行优化。结果表明,哈维氏弧菌qnr全长651 bp,编码216个氨基酸;重组蛋白优化条件为于28℃、IPTG浓度为0.05 mmol/L条件下诱导6 h。

关键词(KeyWords)： 哈维氏弧菌;qnr基因;原核表达;优化

Abstract:

Keywords:

基金项目(Foundation): 农业部行业专项,渔药使用风险评估及其控制技术研究与示范(201203085);; 广东省教育厅高等学校高层次人才项目(谷胱甘肽及其合成酶系在哈氏弧菌耐药中的作用机制研究)

作者(Author): 周维,汤菊芬,高增鸿,甘桢,简纪常,吴灶和,丁燏

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