王忠良,简纪常,鲁义善,吴灶和

• 1:广东海洋大学水产经济动物病原生物学及流行病学重点实验室
• 2:广东海洋大学南海水产经济动物增养殖重点实验室
• 3:广东海洋大学水产学院

摘要(Abstract)：

在构建马氏珠母贝(Pinctada fucata)血淋巴抑制性差减文库的基础上,应用cDNA末端快速扩增(RACE)技术成功克隆马氏珠母贝多聚泛素基因(PfUb)最后一个重复单元及3'端非编码区(UTR)序列。序列长453 bp,其中3′UTR 219 bp;开放阅读框(ORF)234 bp,编码77个氨基酸残基;PfUb重复单元含有7个Lys和1个76Gly泛素基因功能位点。实时荧光定量PCR分析表明,PfUb基因在马氏珠母贝足、外套膜、鳃、闭壳肌、心脏、消化腺、血淋巴7个组织中均有表达,溶藻弧菌(Vibrio alginolyticus)能够诱导马氏珠母贝各组织中PfUb基因的表达上调,其中以鳃和血淋巴中的上调表达最为明显;马氏珠母贝血淋巴中,PfUb基因在溶藻弧菌感染2 h后表达量开始上调,并在感染8 h后达到最大。

关键词(KeyWords)： 马氏珠母贝;多聚泛素;基因;重复单元;非编码区;克隆;表达;RACE;RT-PCR

Abstract:

Keywords:

基金项目(Foundation): 国家星火计划“南海水产动物病害防控技术应用示范”(2008GA780013)

作者(Author): 王忠良,简纪常,鲁义善,吴灶和

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