董佳昱,田姗姗,钱丽,姜苏,唐云平

• 1:浙江海洋大学食品与药学学院
• 2:浙江省海洋生物医用制品工程技术研究中心
• 3:上海欧睿生物科技有限公司

摘要(Abstract)：

【目的】研究从大西洋鲱（Clupea harengus）鱼籽中提取的富二十二碳六烯酸-磷脂酰胆碱（DHA-PC）对双酚S(BPS)诱导的小鼠肝损伤的改善作用。【方法】将24只雄性C57BL/6小鼠随机分成对照组、BPS模型组和DHA-PC给药组，每组8只，模型组和给药组分别给予5 mg·（kg·d）-1BPS，给药组同时灌胃80 mg·（kg·d）~(-1)DHAPC，饲养10周。监测各组小鼠的食物摄入量、体质量、肝功能、附睾脂肪指数、肝脏组织病理学分析、肝脏组织中炎症因子和氧化应激等指标，研究DHA-PC对BPS诱导的小鼠肝损伤的改善作用。【结果】BPS处理后，模型组小鼠体质量、肝脏指数、附睾脂肪指数显著升高（P <0.05），但经DHA-PC饲喂治疗后，均呈现下降趋势。所有组别小鼠的食物摄入量、能量摄入量无差异（P> 0.05）。病理学结果显示模型组小鼠肝脏肝索消失，肝脏发生局灶性脂肪性病变，给药组肝索恢复，中央静脉重现。与模型组相比，DHA-PC给药组显著降低谷丙转氨酶（ALT）、谷草转氨酶（AST）、白介素（IL）-1β、IL-6、肿瘤坏死因子（TNF）-α、胆固醇（TC）、甘油三酯（TG）、游离脂肪酸（NEFA）、低密度脂蛋白（LDL-C）和丙二醛（MDA）的含量（P <0.05），显著提高高密度脂蛋白（HDL-C）、总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和过氧化氢酶（CAT）的含量（P <0.05）。【结论】鲱鱼鱼籽源DHA-PC可通过抑制炎症、改善脂质代谢紊乱和氧化应激等途径有效改善BPS诱导的小鼠肝损伤。

关键词(KeyWords)： 二十二碳六烯酸-磷脂酰胆碱;双酚S;小鼠;肝损伤

Abstract:

Keywords:

基金项目(Foundation): 浙江省基础公益研究计划项目（LTGD23D060001）

作者(Author): 董佳昱,田姗姗,钱丽,姜苏,唐云平

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