陈付菊,付生云,令小东,常兰,李雪源

• 1:青海大学农牧学院
• 2:青海省裸鲤救助中心

摘要(Abstract)：

【目的】研究青海湖裸鲤在低氧胁迫下肌肉组织线粒体呼吸链复合体酶活性及抗氧化酶活性的变化规律,探讨其对低氧的应答机制。【方法】取体质量为(97.68±0.12)g的青海湖裸鲤,分别于中、重度低氧[溶解氧分别为(3.0±0.1)、(0.7±0.1) mg/L]条件下胁迫8、24 h,观察肌肉组织线粒体形态结构,检测线粒体呼吸链复合体Ⅰ-Ⅳ酶活性及抗氧化酶活性。【结果】1)重度低氧胁迫使肌肉组织线粒体长径较常氧对照组大(P <0.05),短径在胁迫8 h时显著增大(P <0.05),胁迫24 h与对照组无显著差异(P> 0.05);部分线粒体嵴溶解,线粒体出现空泡化。中度低氧胁迫组长径和短径与常氧组无显著差异(P> 0.05),未见空泡形成。2)线粒体呼吸链复合体Ⅰ和Ⅲ活性在重度低氧胁迫8 h时显著增加(P <0.05),24 h时降至常氧水平,二者在中度低氧胁迫24 h时显著增加(P <0.05);复合体Ⅱ在各组之间均无显著变化(P> 0.05);复合体Ⅳ在重度低氧胁迫后与常氧组之间无显著差异,而在中度低氧胁迫24 h时显著增加(P <0.05)。3)过氧化氢(H2O2)含量在重度低氧胁迫8 h时显著增加(P <0.05),24 h时降至常氧水平,中度低氧胁迫24 h时与常氧组之间无显著差异(P> 0.05);丙二醛(MDA)含量在重度和中度低氧胁迫后显著增加(P <0.05);超氧化物歧化酶(SOD)活性在重度低氧胁迫后与常氧组之间无显著差异,而在中度低氧胁迫24 h时显著增加(P <0.05);总抗氧化能力(T-AOC)和谷胱甘肽过氧化物酶(GPX)活性在重度和中度低氧胁迫后显著增加(P <0.05)。【结论】青海湖裸鲤肌肉组织对溶氧骤变产生应答反应,通过调整线粒体体积、形状结构、呼吸链复合体酶活性及相关抗氧化酶活性提高其低氧适应能力。

关键词(KeyWords)： 青海湖裸鲤;低氧胁迫;肌肉组织;线粒体呼吸链复合体活性;抗氧化酶活性

Abstract:

Keywords:

基金项目(Foundation): 青海省科技厅应用基础研究项目（2018-ZJ-729）

作者(Author): 陈付菊,付生云,令小东,常兰,李雪源

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