冯建儒,范晨龙,丁燏

• 1:广东海洋大学水产学院//广东省水产经济动物病原生物学及流行病学重点实验室//水产经济动物病害防控广东普通高校重点实验室

摘要(Abstract)：

【目的】研究敲除谷胱甘肽还原酶(GR)基因gr对溶藻弧菌HY9901生物学特性的影响。【方法】通过同源重组技术和Overlap PCR等分子生物学手段构建溶藻弧菌HY9901的gr基因缺失株,通过反转录验证并检测其遗传稳定性,绘制该菌的野生株和缺失株的生长曲线,测定该菌野生株和缺失株的生物膜形成能力、抗生素敏感性、胞外蛋白酶活性和泳动能力等生物学特性。【结果】gr基因的缺失几乎不影响溶藻弧菌的生长趋势,但敲除gr基因后溶藻弧菌对呋喃唑酮、头孢唑林、四环素等敏感性增强,该菌的生物膜形成能力增强显著提高。缺失株△gr的胞外蛋白酶活性和泳动能力均低于野生株。【结论】谷胱甘肽还原酶在溶藻弧菌的毒力、生物膜形成、药物敏感性、泳动等方面有重要作用。

关键词(KeyWords)： 溶藻弧菌;谷胱甘肽还原酶;缺失株;生物学特性;同源重组

Abstract:

Keywords:

基金项目(Foundation): 广东自然科学基金（2014A030313604）;; 广东海洋大学研究生教育创新计划资助项目（201724）

作者(Author): 冯建儒,范晨龙,丁燏

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