孙宗红,刘志刚,白成,李健强,刘超

• 1:广东海洋大学水产学院

摘要(Abstract)：

谷胱甘肽过氧化物酶(GPX)基因表达可反映生物对环境压力的抗逆能力。采用中心复合设计和响应曲面分析法研究不同温度(18~34℃)、盐度(20~40)对4种壳色马氏珠母贝GPX基因表达量的影响。结果表明,马氏珠母贝4种壳色群体GPX基因表达量随着温度和盐度的升高,呈现先降低后升高的趋势。温度的一次、二次效应对4种壳色马氏珠母贝GPX基因表达的影响有统计学意义(P<0.01)。盐度的一次效应仅对黑壳色马氏珠母贝GPX基因表达影响有统计学意义(P<0.05);盐度的二次效应对白壳色马氏珠母贝GPX基因表达的影响有统计学意义(P<0.05),对黑、红、黄壳色马氏珠母贝GPX基因表达的影响有统计学意义(P<0.01)。温盐协同效应对黑、红、黄壳色马氏珠母贝GPX基因表达的影响有统计学意义(P<0.05),且温度的效应大于盐度效应。经响应曲面分析法和优化,得到马氏珠母贝的GPX基因表达量在适宜的温度、盐度范围内呈峰值变化,白、黑、红、黄等4种壳色马氏珠母贝分别在(21.4℃,30.1)、(24.8℃,28.5)、(25.2℃,30.5)、(25.1℃,29.7)等温盐度组合条件下达到最优(低)值(0.072、0.065、0.057、0.078),满意度分别为100%、100%、97%、100%。所建立回归模型方程的决定系数R2范围为0.971 3~0.995 8,校正决定系数R2范围为0.942 6~0.991 5,预测决定系数R2范围为0.713 9~0.968 0,模型拟合度高,模型恰当,可用于预测温度、盐度对4种壳色马氏珠母贝GPX基因表达量的影响。

关键词(KeyWords)： 马氏珠母贝;谷胱甘肽过氧化物酶;基因表达;温度;盐度;响应曲面法

Abstract:

Keywords:

基金项目(Foundation): 广东省海洋渔业科技推广专项(A201108A04)

作者(Author): 孙宗红,刘志刚,白成,李健强,刘超

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