DABS-Cl柱前衍生高效液相色谱法测定对虾肌肉中18种游离氨基酸Determination of 18 Free Amino Acids in the Muscle of Shrimp by Reversed-Phase High Performance Liquid Chromatography with DABS-Cl Precolumn Derivatization
吴成福,林黑着,李刘冬,江世贵
摘要(Abstract)：
建立一种以DABS-Cl为柱前衍生剂,利用反相高效液相色谱测定对虾肌肉中18种游离氨基酸的方法。结果表明,在50 min内18种氨基酸得到完全分离,各氨基酸相关系数均大于0.997,氨基酸的回收率在86.60%~107.27%之间(胱氨酸除外),方法的检出限为0.18~0.66 mg/L,相对标准偏差为3.53%~8.51%(天冬氨酸除外)。
关键词(KeyWords)： 对虾肌肉;游离氨基酸;高效液相色谱法;柱前衍生剂;DABS-Cl
基金项目(Foundation): 863项目(2003AA60312);; 农业结构调整项目(06-05-018)
作者(Author): 吴成福,林黑着,李刘冬,江世贵
参考文献(References)：
- [1]Shahidi F.肉制品与水产品风味[M].李洁,朱国斌,译.第2版.北京:中国轻工业出版社,2001:129-164.
- [2]杜文力,孟冲,史志冲.高效液相色谱法(HPLC)分析氨基酸概况[J].河北医药,2004,26(1):69-70.
- [3]于泓,牟世芬.氨基酸分析方法的研究进展[J].分析化学,2005,33(3):398-404.
- [4]宋志峰,王丽,纪锋,等.氨基酸分析中的柱前衍生技术[J].吉林农业科学,2004,29(6):54-58.
- [5]Romero R,Ga′zquez D,Bagur M G,Sa′nchez-vinas M.Optimization of chromatographic parameters for the determination of biogenic amines in wines by reversed-phase high-performance liquid chromato-graphy[J].Journal of Chromatography A,2000,871:75-83.
- [6]Olivia P,Isable M F,Eulalia M,et al.Effect of temperature on evolution of free amino acid and biogenic amine contents during storage of Azeit?o cheese[J].Food chemistry,2001,75:287-291.
- [7]Krause I,Bockhardt A,Neckermann H,et al.Simultaneous determi-nation of amino acids and biogenic amines by reversed-phase high-performance liquid chromatography of the dabsyl derivatives[J].Journal of Chromatography A,1995,715:67-79.
- [8]曲宁,周燕,顾履珍,等.粮食中氨基酸HPLC测定方法的研究[J].卫生研究,1997,26(6):415-417.
- [9]费达,江国荣,李勇健,等.DABS-Cl试剂在反相高效液相色谱中的应用[J].化学试剂,1993,15(1):42-44.
- [10]张露蓉,江国荣.DABS-Cl柱前衍生RP-HPLC法定量测定血清中游离氨基酸[J].苏州大学学报(自然科学版),2004,20(3):65-71.
- [11]Alpdogan G.Determination of homocystine as dabsyl derivative by HPLC using precolumn derivatization[J].Analytical letters,2001,33(13):2645-2651.