周建淑,史策,叶央芳,王春琳,母昌考,李荣华,刘海娟,吴清洋,王晓朋,周月越,陈书健

• 1:宁波大学水产生物技术教育部重点实验室/宁波大学农业农村部绿色海水养殖重点实验室(省部共建)
• 2:广西海洋研究所有限责任公司

摘要(Abstract)：

【目的】探究拟穴青蟹（Scylla paramamosain）循环水养殖系统（Recirculating aquaculture systems,RAS）生物滤池启动过程中细菌群落的演替过程，为设计适合拟穴青蟹RAS养殖的生物过滤器提供指导。【方法】在2、5、10、20、30、40、50 d检测RAS养殖池的水质，采集填料生物膜和养殖池水体微生物样品，采用多种生物信息学分析方法研究生物膜微生物群落演替过程，并运用实时荧光定量PCR技术检测生物膜碳循环和氮循环功能基因拷贝数。【结果与结论】氨氮（NH_4~+-N）、分子氨（NH_3）和亚硝态氮（NO_2~--N）的质量浓度总体呈先上升后下降趋势，硝态氮（NO_3~--N）和总氮（TN）在实验结束时（50 d）积累，总有机碳（TOC）质量浓度在前10 d保持稳定，从10 d至50 d呈先升高后下降趋势，差异显著（P<0.05），且总氮和硝态氮质量浓度对生物膜细菌群落变化的影响最大。生物膜细菌群落的多样性、均匀性和丰富度在10 d达到峰值，然后达到平稳状态。基于FAPROTAX的功能预测发现生物膜大多数功能主要富集在氮循环和有机物降解这两方面，生物膜碳循环和氮循环绝对基因拷贝数随时间增加总体呈升高趋势。根据FEAST分析结果，除10 d以外，前20 d同一时期的水体细菌对生物膜的贡献较高，后30 d主要来源于生物膜本身前期细菌贡献，且贡献率随时间增加呈增长趋势，到后期趋于稳定。结果表明，拟穴青蟹RAS生物滤池启动过程中，生物膜的细菌群落结构随时间增加变化显著，功能主要富集在氮循环和有机物降解这两方面，生物膜细菌来自本身细菌的贡献率也逐渐升高，到后期趋于稳定。

关键词(KeyWords)： 拟穴青蟹;循环水养殖系统;生物膜;细菌群落;水质;功能基因

Abstract:

Keywords:

基金项目(Foundation): 宁波市国际合作项目（2023H012）;; 农业农村部国家虾蟹产业技术体系资助（CARS-48）

作者(Author): 周建淑,史策,叶央芳,王春琳,母昌考,李荣华,刘海娟,吴清洋,王晓朋,周月越,陈书健

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