翟毅,刘建业,贾丽英,江东能,陈华谱,李广丽,邓思平

• 1:广东海洋大学水产学院
• 2:广东省名特优鱼类生殖调控与繁育工程技术研究中心
• 3:海洋生态与养殖环境湛江市重点实验室

摘要(Abstract)：

【目的】克隆金钱鱼cyp17a1基因,分析其在组织中的分布、卵巢不同发育时期中的表达变化,为金钱鱼繁殖生物学提供理论依据。【方法】通过金钱鱼转录组文库筛选和RT-PCR进行cyp17a1 c DNA序列扩增;用DNAstar软件比较Cyp17a1同源性,MEGA5.0构建系统进化树,RT-PCR检测cyp17a1组织表达,实时定量PCR检测不同卵巢发育时期cyp17a1的表达量。【结果】金钱鱼cyp17a1开放阅读框编码515个氨基酸残基;Cyp17a1氨基酸序列与同属鲈形目鱼类Cyp17a1同源性较高(87.8%～92.7%),与大黄鱼最高(92.7%),与其他目鱼类同源性次之(72.7%～85.0%),与硬骨鱼类Cyp17a2或Cyp17a1-like同源性最低(48.2%～51.7%);所有硬骨鱼类Cyp17a1聚为一支,在Cyp17a1分支中,金钱鱼与鲈形目鱼类聚为一支;cyp17a1在性腺中表达量较高,在精巢中表达最强;在肝、脑和头肾表达较弱;在脾脏、肠、心脏、肌肉和鳃中未检测到表达;卵巢中cyp17a1的表达量随卵巢发育逐渐增加,Ⅳ期卵巢中cyp17a1表达量最高。【结论】cyp17a1在性腺中表达量较高,在金钱鱼卵巢发育过程中有重要作用。

关键词(KeyWords)： 金钱鱼;cyp17a1;基因克隆;组织分布;基因表达;卵巢发育

Abstract:

Keywords:

基金项目(Foundation): 大学生创新创业计划项目(201610566007,201410566046);; 广东省自然科学基金(2016A030313743,2017A030313101);; 广东省海洋与渔业局科技攻关与研发及技术推广项目(A201608B01,2017A0012);; 国家自然科学基金项目(Nos.41706174,31702326);; 湛江市科技攻关计划项目(2016A03017)

作者(Author): 翟毅,刘建业,贾丽英,江东能,陈华谱,李广丽,邓思平

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